TY - JOUR
T1 - Angle and locus of the bend induced by the MspI DNA methyltransferase in a sequence-specific complex with DNA
AU - Dubey, Ashok K.
AU - Bhattacharya, Sanjoy K.
PY - 1997
Y1 - 1997
N2 - Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.
AB - Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.
UR - http://www.scopus.com/inward/record.url?scp=0030842058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030842058&partnerID=8YFLogxK
U2 - 10.1093/nar/25.10.2025
DO - 10.1093/nar/25.10.2025
M3 - Article
C2 - 9115372
AN - SCOPUS:0030842058
VL - 25
SP - 2025
EP - 2029
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 10
ER -