Angle and locus of the bend induced by the MspI DNA methyltransferase in a sequence-specific complex with DNA

Ashok K. Dubey; Sanjoy K. Bhattacharya

doi:10.1093/nar/25.10.2025

Angle and locus of the bend induced by the MspI DNA methyltransferase in a sequence-specific complex with DNA

Ashok K. Dubey, Sanjoy K. Bhattacharya

Ophthalmology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.

Original language	English (US)
Pages (from-to)	2025-2029
Number of pages	5
Journal	Nucleic acids research
Volume	25
Issue number	10
DOIs	https://doi.org/10.1093/nar/25.10.2025
State	Published - 1997

ASJC Scopus subject areas

Genetics

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title = "Angle and locus of the bend induced by the MspI DNA methyltransferase in a sequence-specific complex with DNA",

abstract = "Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.",

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T1 - Angle and locus of the bend induced by the MspI DNA methyltransferase in a sequence-specific complex with DNA

AU - Dubey, Ashok K.

AU - Bhattacharya, Sanjoy K.

PY - 1997

Y1 - 1997

N2 - Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.

AB - Bending of DNA induced by M.MspI, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.MspI MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3' which was estimated to be 142 ± 4°and 132 ± 4°for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of ~ 15 kcal/mol was obtained for the free energy associated with M.MspI-induced DNA bending.

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

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