Androgen Receptor Expression and DNA Content of Paraffin-Embedded Archival Human Prostate Tumors

Satish Kumar Adiga, Ilia Andritsch, Ravikala Vittal Rao, Awtar Krishan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Androgen receptors (AR) are expressed in human prostate cells and immunohistochemistry has been used for qualitative analysis of AR expression in prostate tumor cells. Quantitative and multiparametric analysis of receptor expression could be of diagnostic and prognostic value in the management of patients on antiandrogen therapy. Multiparametric flow cytometric methods have been developed for analysis of hormone receptor expression and DNA content in nuclei isolated from formalin-fixed/paraffin-embedded human solid tumors. The present study was undertaken for analysis of AR expression and DNA content in archival human prostate tumors. Methods: AR expression and DNA content were measured in nuclei isolated by enzyme digestion from thick sections cut from 51 paraffin-embedded human prostate tumors. AR expression in different subpopulations was studied by gated analysis. The relationship among AR activity, DNA content, and histopathological grade was analyzed. Results: Distinct aneuploid populations were observed in 23% of tumors examined. AR activity was observed in all the specimens and the percentage of AR- positive nuclei in the 48 samples analyzed was < 10% (n = 4), 11-50% (n = 39), and >51% (n = 5). Tumor subpopulations with aneuploid DNA content had higher AR expression (percent AR-positive cells and mean log fluorescence) than the diploid subpopulations. No strong correlation was seen between AR expression and histopathological grade of the tumors. Conclusions: Flow cytometric analysis of archival prostate tumor can be used for rapid determination of aneuploid DNA content and AR expression in subpopulations of nuclei isolated from formalin-fixed/paraffin-embedded prostate tumor blocks.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalClinical Cytometry
Volume50
Issue number1
DOIs
StatePublished - Feb 15 2002
Externally publishedYes

Fingerprint

Androgen Receptors
Paraffin
Prostate
DNA
Neoplasms
Aneuploidy
Formaldehyde
Androgen Antagonists
Diploidy
Digestion
Fluorescence
Immunohistochemistry
Hormones

Keywords

  • Androgen receptors
  • DNA ploidy
  • Flow cytometry
  • Prostate cancer

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Androgen Receptor Expression and DNA Content of Paraffin-Embedded Archival Human Prostate Tumors. / Adiga, Satish Kumar; Andritsch, Ilia; Rao, Ravikala Vittal; Krishan, Awtar.

In: Clinical Cytometry, Vol. 50, No. 1, 15.02.2002, p. 25-30.

Research output: Contribution to journalArticle

Adiga, Satish Kumar ; Andritsch, Ilia ; Rao, Ravikala Vittal ; Krishan, Awtar. / Androgen Receptor Expression and DNA Content of Paraffin-Embedded Archival Human Prostate Tumors. In: Clinical Cytometry. 2002 ; Vol. 50, No. 1. pp. 25-30.
@article{63080c3a357247e196b487912dc12afc,
title = "Androgen Receptor Expression and DNA Content of Paraffin-Embedded Archival Human Prostate Tumors",
abstract = "Background: Androgen receptors (AR) are expressed in human prostate cells and immunohistochemistry has been used for qualitative analysis of AR expression in prostate tumor cells. Quantitative and multiparametric analysis of receptor expression could be of diagnostic and prognostic value in the management of patients on antiandrogen therapy. Multiparametric flow cytometric methods have been developed for analysis of hormone receptor expression and DNA content in nuclei isolated from formalin-fixed/paraffin-embedded human solid tumors. The present study was undertaken for analysis of AR expression and DNA content in archival human prostate tumors. Methods: AR expression and DNA content were measured in nuclei isolated by enzyme digestion from thick sections cut from 51 paraffin-embedded human prostate tumors. AR expression in different subpopulations was studied by gated analysis. The relationship among AR activity, DNA content, and histopathological grade was analyzed. Results: Distinct aneuploid populations were observed in 23{\%} of tumors examined. AR activity was observed in all the specimens and the percentage of AR- positive nuclei in the 48 samples analyzed was < 10{\%} (n = 4), 11-50{\%} (n = 39), and >51{\%} (n = 5). Tumor subpopulations with aneuploid DNA content had higher AR expression (percent AR-positive cells and mean log fluorescence) than the diploid subpopulations. No strong correlation was seen between AR expression and histopathological grade of the tumors. Conclusions: Flow cytometric analysis of archival prostate tumor can be used for rapid determination of aneuploid DNA content and AR expression in subpopulations of nuclei isolated from formalin-fixed/paraffin-embedded prostate tumor blocks.",
keywords = "Androgen receptors, DNA ploidy, Flow cytometry, Prostate cancer",
author = "Adiga, {Satish Kumar} and Ilia Andritsch and Rao, {Ravikala Vittal} and Awtar Krishan",
year = "2002",
month = "2",
day = "15",
doi = "10.1002/cyto.10050",
language = "English",
volume = "50",
pages = "25--30",
journal = "Cytometry Part B - Clinical Cytometry",
issn = "1552-4949",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Androgen Receptor Expression and DNA Content of Paraffin-Embedded Archival Human Prostate Tumors

AU - Adiga, Satish Kumar

AU - Andritsch, Ilia

AU - Rao, Ravikala Vittal

AU - Krishan, Awtar

PY - 2002/2/15

Y1 - 2002/2/15

N2 - Background: Androgen receptors (AR) are expressed in human prostate cells and immunohistochemistry has been used for qualitative analysis of AR expression in prostate tumor cells. Quantitative and multiparametric analysis of receptor expression could be of diagnostic and prognostic value in the management of patients on antiandrogen therapy. Multiparametric flow cytometric methods have been developed for analysis of hormone receptor expression and DNA content in nuclei isolated from formalin-fixed/paraffin-embedded human solid tumors. The present study was undertaken for analysis of AR expression and DNA content in archival human prostate tumors. Methods: AR expression and DNA content were measured in nuclei isolated by enzyme digestion from thick sections cut from 51 paraffin-embedded human prostate tumors. AR expression in different subpopulations was studied by gated analysis. The relationship among AR activity, DNA content, and histopathological grade was analyzed. Results: Distinct aneuploid populations were observed in 23% of tumors examined. AR activity was observed in all the specimens and the percentage of AR- positive nuclei in the 48 samples analyzed was < 10% (n = 4), 11-50% (n = 39), and >51% (n = 5). Tumor subpopulations with aneuploid DNA content had higher AR expression (percent AR-positive cells and mean log fluorescence) than the diploid subpopulations. No strong correlation was seen between AR expression and histopathological grade of the tumors. Conclusions: Flow cytometric analysis of archival prostate tumor can be used for rapid determination of aneuploid DNA content and AR expression in subpopulations of nuclei isolated from formalin-fixed/paraffin-embedded prostate tumor blocks.

AB - Background: Androgen receptors (AR) are expressed in human prostate cells and immunohistochemistry has been used for qualitative analysis of AR expression in prostate tumor cells. Quantitative and multiparametric analysis of receptor expression could be of diagnostic and prognostic value in the management of patients on antiandrogen therapy. Multiparametric flow cytometric methods have been developed for analysis of hormone receptor expression and DNA content in nuclei isolated from formalin-fixed/paraffin-embedded human solid tumors. The present study was undertaken for analysis of AR expression and DNA content in archival human prostate tumors. Methods: AR expression and DNA content were measured in nuclei isolated by enzyme digestion from thick sections cut from 51 paraffin-embedded human prostate tumors. AR expression in different subpopulations was studied by gated analysis. The relationship among AR activity, DNA content, and histopathological grade was analyzed. Results: Distinct aneuploid populations were observed in 23% of tumors examined. AR activity was observed in all the specimens and the percentage of AR- positive nuclei in the 48 samples analyzed was < 10% (n = 4), 11-50% (n = 39), and >51% (n = 5). Tumor subpopulations with aneuploid DNA content had higher AR expression (percent AR-positive cells and mean log fluorescence) than the diploid subpopulations. No strong correlation was seen between AR expression and histopathological grade of the tumors. Conclusions: Flow cytometric analysis of archival prostate tumor can be used for rapid determination of aneuploid DNA content and AR expression in subpopulations of nuclei isolated from formalin-fixed/paraffin-embedded prostate tumor blocks.

KW - Androgen receptors

KW - DNA ploidy

KW - Flow cytometry

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=0037085420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037085420&partnerID=8YFLogxK

U2 - 10.1002/cyto.10050

DO - 10.1002/cyto.10050

M3 - Article

VL - 50

SP - 25

EP - 30

JO - Cytometry Part B - Clinical Cytometry

JF - Cytometry Part B - Clinical Cytometry

SN - 1552-4949

IS - 1

ER -