Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors

Awtar Krishan, Poonam Arya, Parvin Ganjei-Azar, Suzanne E. Shirley, Carlos T. Escoffery, Mehrdad Nadji

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: The present study was undertaken for quantitation of androgen (AR) and vitamin D (VDR) receptor expression in human male and female breast tumors by flow cytometry. Methods: Nuclei isolated from sections of paraffin-embedded tumors by pepsin digestion were treated for antigen unmasking and incubated with antibodies to AR and VDR. Flow cytometric analysis was used to determine the percentage of receptor-positive nuclei with fluorescence greater than 95% of the isotype nuclei. Mean log fluorescence channel values were used for comparing antigen density of the isotype and the antibody-treated nuclei. Results. Six of 23 female breast tumors had aneuploid DNA content. Nineteen of 20 estrogen receptor-positive female tumors by immunohistochemical analysis (IHC) were also AR positive by flow analysis. Aneuploid subpopulations had higher percentages of AR-positive nuclei than did diploid populations. Eight of 33 male breast tumors had aneuploid DNA content. Twenty-three of 33 male breast tumors were AR positive by flow analysis compared with six that were AR positive by IHC. Six AR-positive (IHC) male tumors were also AR positive by flow analysis. VDR expression was higher in diploid female tumors than in aneuploid tumors. Conclusions: Lack of a strong correlation between IHC and flow analysis may be due to differences in criteria used for identification of receptor-positive and -negative tumors by the two methods.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalCytometry Part B - Clinical Cytometry
Volume58
Issue number1
StatePublished - Mar 1 2004

Fingerprint

Calcitriol Receptors
Androgens
Breast Neoplasms
Aneuploidy
Male Breast Neoplasms
Neoplasms
Diploidy
Fluorescence
Antigens
Antibodies
Pepsin A
DNA
Estrogen Receptors
Paraffin
Digestion
Flow Cytometry

Keywords

  • Androgen receptor
  • Female breast tumor
  • Flow cytometry
  • Male breast tumor
  • Vitamin D receptor

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Krishan, A., Arya, P., Ganjei-Azar, P., Shirley, S. E., Escoffery, C. T., & Nadji, M. (2004). Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors. Cytometry Part B - Clinical Cytometry, 58(1), 53-60.

Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors. / Krishan, Awtar; Arya, Poonam; Ganjei-Azar, Parvin; Shirley, Suzanne E.; Escoffery, Carlos T.; Nadji, Mehrdad.

In: Cytometry Part B - Clinical Cytometry, Vol. 58, No. 1, 01.03.2004, p. 53-60.

Research output: Contribution to journalArticle

Krishan, A, Arya, P, Ganjei-Azar, P, Shirley, SE, Escoffery, CT & Nadji, M 2004, 'Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors', Cytometry Part B - Clinical Cytometry, vol. 58, no. 1, pp. 53-60.
Krishan A, Arya P, Ganjei-Azar P, Shirley SE, Escoffery CT, Nadji M. Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors. Cytometry Part B - Clinical Cytometry. 2004 Mar 1;58(1):53-60.
Krishan, Awtar ; Arya, Poonam ; Ganjei-Azar, Parvin ; Shirley, Suzanne E. ; Escoffery, Carlos T. ; Nadji, Mehrdad. / Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors. In: Cytometry Part B - Clinical Cytometry. 2004 ; Vol. 58, No. 1. pp. 53-60.
@article{7bcdcf96a5fa4d51b24ae916fe537cd5,
title = "Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors",
abstract = "Background: The present study was undertaken for quantitation of androgen (AR) and vitamin D (VDR) receptor expression in human male and female breast tumors by flow cytometry. Methods: Nuclei isolated from sections of paraffin-embedded tumors by pepsin digestion were treated for antigen unmasking and incubated with antibodies to AR and VDR. Flow cytometric analysis was used to determine the percentage of receptor-positive nuclei with fluorescence greater than 95{\%} of the isotype nuclei. Mean log fluorescence channel values were used for comparing antigen density of the isotype and the antibody-treated nuclei. Results. Six of 23 female breast tumors had aneuploid DNA content. Nineteen of 20 estrogen receptor-positive female tumors by immunohistochemical analysis (IHC) were also AR positive by flow analysis. Aneuploid subpopulations had higher percentages of AR-positive nuclei than did diploid populations. Eight of 33 male breast tumors had aneuploid DNA content. Twenty-three of 33 male breast tumors were AR positive by flow analysis compared with six that were AR positive by IHC. Six AR-positive (IHC) male tumors were also AR positive by flow analysis. VDR expression was higher in diploid female tumors than in aneuploid tumors. Conclusions: Lack of a strong correlation between IHC and flow analysis may be due to differences in criteria used for identification of receptor-positive and -negative tumors by the two methods.",
keywords = "Androgen receptor, Female breast tumor, Flow cytometry, Male breast tumor, Vitamin D receptor",
author = "Awtar Krishan and Poonam Arya and Parvin Ganjei-Azar and Shirley, {Suzanne E.} and Escoffery, {Carlos T.} and Mehrdad Nadji",
year = "2004",
month = "3",
day = "1",
language = "English",
volume = "58",
pages = "53--60",
journal = "Cytometry Part B - Clinical Cytometry",
issn = "1552-4949",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Androgen and Vitamin D Receptor Expression in Archival Human Breast Tumors

AU - Krishan, Awtar

AU - Arya, Poonam

AU - Ganjei-Azar, Parvin

AU - Shirley, Suzanne E.

AU - Escoffery, Carlos T.

AU - Nadji, Mehrdad

PY - 2004/3/1

Y1 - 2004/3/1

N2 - Background: The present study was undertaken for quantitation of androgen (AR) and vitamin D (VDR) receptor expression in human male and female breast tumors by flow cytometry. Methods: Nuclei isolated from sections of paraffin-embedded tumors by pepsin digestion were treated for antigen unmasking and incubated with antibodies to AR and VDR. Flow cytometric analysis was used to determine the percentage of receptor-positive nuclei with fluorescence greater than 95% of the isotype nuclei. Mean log fluorescence channel values were used for comparing antigen density of the isotype and the antibody-treated nuclei. Results. Six of 23 female breast tumors had aneuploid DNA content. Nineteen of 20 estrogen receptor-positive female tumors by immunohistochemical analysis (IHC) were also AR positive by flow analysis. Aneuploid subpopulations had higher percentages of AR-positive nuclei than did diploid populations. Eight of 33 male breast tumors had aneuploid DNA content. Twenty-three of 33 male breast tumors were AR positive by flow analysis compared with six that were AR positive by IHC. Six AR-positive (IHC) male tumors were also AR positive by flow analysis. VDR expression was higher in diploid female tumors than in aneuploid tumors. Conclusions: Lack of a strong correlation between IHC and flow analysis may be due to differences in criteria used for identification of receptor-positive and -negative tumors by the two methods.

AB - Background: The present study was undertaken for quantitation of androgen (AR) and vitamin D (VDR) receptor expression in human male and female breast tumors by flow cytometry. Methods: Nuclei isolated from sections of paraffin-embedded tumors by pepsin digestion were treated for antigen unmasking and incubated with antibodies to AR and VDR. Flow cytometric analysis was used to determine the percentage of receptor-positive nuclei with fluorescence greater than 95% of the isotype nuclei. Mean log fluorescence channel values were used for comparing antigen density of the isotype and the antibody-treated nuclei. Results. Six of 23 female breast tumors had aneuploid DNA content. Nineteen of 20 estrogen receptor-positive female tumors by immunohistochemical analysis (IHC) were also AR positive by flow analysis. Aneuploid subpopulations had higher percentages of AR-positive nuclei than did diploid populations. Eight of 33 male breast tumors had aneuploid DNA content. Twenty-three of 33 male breast tumors were AR positive by flow analysis compared with six that were AR positive by IHC. Six AR-positive (IHC) male tumors were also AR positive by flow analysis. VDR expression was higher in diploid female tumors than in aneuploid tumors. Conclusions: Lack of a strong correlation between IHC and flow analysis may be due to differences in criteria used for identification of receptor-positive and -negative tumors by the two methods.

KW - Androgen receptor

KW - Female breast tumor

KW - Flow cytometry

KW - Male breast tumor

KW - Vitamin D receptor

UR - http://www.scopus.com/inward/record.url?scp=1542723663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542723663&partnerID=8YFLogxK

M3 - Article

C2 - 14994376

AN - SCOPUS:1542723663

VL - 58

SP - 53

EP - 60

JO - Cytometry Part B - Clinical Cytometry

JF - Cytometry Part B - Clinical Cytometry

SN - 1552-4949

IS - 1

ER -