Ancient bone collagen assessment by hand-held vibrational spectroscopy

William Pestle, Fakhra Ahmad, Benjamin J. Vesper, Geoffrey A. Cordell, Michael D. Colvard

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Radiometric, isotopic, proteomic, and genetic studies of bone collagen are mainstays of archaeometric analysis. All four techniques are destructive and require substantial laboratory, temporal, and financial commitments. Because such analyses are predicated on the presence of a sufficient quantity of unaltered proteins (i.e. collagen), the development and validation of tools for the rapid, non-destructive, in situ analysis of collagen content could yield measurable benefits. In the present work, the results of a preliminary, proof-of-concept study on the utility of four hand-held vibrational spectroscopic instruments, one Fourier-Transform Infrared (FTIR) spectrometer and three Raman spectrometers (two with an excitation wavelength of 785nm, and one with an excitation wavelength of 1030nm), for analyzing the collagen content of archaeological bones are described. While the FTIR and 785nm Raman devices showed little or no ability to discriminate between well- and poorly-preserved bone, the application of hand-held 1030nm Raman spectroscopy appears to be well-suited for such a task. The ability to detect a measurable and characteristic spectroscopic peak associated with the δCH2 scissoring of Type I collagen in high-yielding, raw bone samples opens the door to the utilization of this technology in field research environments.

Original languageEnglish (US)
Pages (from-to)381-389
Number of pages9
JournalJournal of Archaeological Science
Volume42
Issue number1
DOIs
StatePublished - Feb 2014

Fingerprint

ability
field research
utilization
commitment
Spectroscopy
Collagen
Bone Collagen
Infrared
Raman
In Situ
Protein
Proteomics
Field Research
Raman Spectroscopy
Archaeological Bone

Keywords

  • ADNA
  • Collagen
  • FTIR
  • Radiocarbon
  • Raman spectroscopy
  • Stable isotope

ASJC Scopus subject areas

  • History
  • Archaeology

Cite this

Ancient bone collagen assessment by hand-held vibrational spectroscopy. / Pestle, William; Ahmad, Fakhra; Vesper, Benjamin J.; Cordell, Geoffrey A.; Colvard, Michael D.

In: Journal of Archaeological Science, Vol. 42, No. 1, 02.2014, p. 381-389.

Research output: Contribution to journalArticle

Pestle, William ; Ahmad, Fakhra ; Vesper, Benjamin J. ; Cordell, Geoffrey A. ; Colvard, Michael D. / Ancient bone collagen assessment by hand-held vibrational spectroscopy. In: Journal of Archaeological Science. 2014 ; Vol. 42, No. 1. pp. 381-389.
@article{51c41a7e1fa548baa17307ba88e79824,
title = "Ancient bone collagen assessment by hand-held vibrational spectroscopy",
abstract = "Radiometric, isotopic, proteomic, and genetic studies of bone collagen are mainstays of archaeometric analysis. All four techniques are destructive and require substantial laboratory, temporal, and financial commitments. Because such analyses are predicated on the presence of a sufficient quantity of unaltered proteins (i.e. collagen), the development and validation of tools for the rapid, non-destructive, in situ analysis of collagen content could yield measurable benefits. In the present work, the results of a preliminary, proof-of-concept study on the utility of four hand-held vibrational spectroscopic instruments, one Fourier-Transform Infrared (FTIR) spectrometer and three Raman spectrometers (two with an excitation wavelength of 785nm, and one with an excitation wavelength of 1030nm), for analyzing the collagen content of archaeological bones are described. While the FTIR and 785nm Raman devices showed little or no ability to discriminate between well- and poorly-preserved bone, the application of hand-held 1030nm Raman spectroscopy appears to be well-suited for such a task. The ability to detect a measurable and characteristic spectroscopic peak associated with the δCH2 scissoring of Type I collagen in high-yielding, raw bone samples opens the door to the utilization of this technology in field research environments.",
keywords = "ADNA, Collagen, FTIR, Radiocarbon, Raman spectroscopy, Stable isotope",
author = "William Pestle and Fakhra Ahmad and Vesper, {Benjamin J.} and Cordell, {Geoffrey A.} and Colvard, {Michael D.}",
year = "2014",
month = "2",
doi = "10.1016/j.jas.2013.11.014",
language = "English (US)",
volume = "42",
pages = "381--389",
journal = "Journal of Archaeological Science",
issn = "0305-4403",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Ancient bone collagen assessment by hand-held vibrational spectroscopy

AU - Pestle, William

AU - Ahmad, Fakhra

AU - Vesper, Benjamin J.

AU - Cordell, Geoffrey A.

AU - Colvard, Michael D.

PY - 2014/2

Y1 - 2014/2

N2 - Radiometric, isotopic, proteomic, and genetic studies of bone collagen are mainstays of archaeometric analysis. All four techniques are destructive and require substantial laboratory, temporal, and financial commitments. Because such analyses are predicated on the presence of a sufficient quantity of unaltered proteins (i.e. collagen), the development and validation of tools for the rapid, non-destructive, in situ analysis of collagen content could yield measurable benefits. In the present work, the results of a preliminary, proof-of-concept study on the utility of four hand-held vibrational spectroscopic instruments, one Fourier-Transform Infrared (FTIR) spectrometer and three Raman spectrometers (two with an excitation wavelength of 785nm, and one with an excitation wavelength of 1030nm), for analyzing the collagen content of archaeological bones are described. While the FTIR and 785nm Raman devices showed little or no ability to discriminate between well- and poorly-preserved bone, the application of hand-held 1030nm Raman spectroscopy appears to be well-suited for such a task. The ability to detect a measurable and characteristic spectroscopic peak associated with the δCH2 scissoring of Type I collagen in high-yielding, raw bone samples opens the door to the utilization of this technology in field research environments.

AB - Radiometric, isotopic, proteomic, and genetic studies of bone collagen are mainstays of archaeometric analysis. All four techniques are destructive and require substantial laboratory, temporal, and financial commitments. Because such analyses are predicated on the presence of a sufficient quantity of unaltered proteins (i.e. collagen), the development and validation of tools for the rapid, non-destructive, in situ analysis of collagen content could yield measurable benefits. In the present work, the results of a preliminary, proof-of-concept study on the utility of four hand-held vibrational spectroscopic instruments, one Fourier-Transform Infrared (FTIR) spectrometer and three Raman spectrometers (two with an excitation wavelength of 785nm, and one with an excitation wavelength of 1030nm), for analyzing the collagen content of archaeological bones are described. While the FTIR and 785nm Raman devices showed little or no ability to discriminate between well- and poorly-preserved bone, the application of hand-held 1030nm Raman spectroscopy appears to be well-suited for such a task. The ability to detect a measurable and characteristic spectroscopic peak associated with the δCH2 scissoring of Type I collagen in high-yielding, raw bone samples opens the door to the utilization of this technology in field research environments.

KW - ADNA

KW - Collagen

KW - FTIR

KW - Radiocarbon

KW - Raman spectroscopy

KW - Stable isotope

UR - http://www.scopus.com/inward/record.url?scp=84890391320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890391320&partnerID=8YFLogxK

U2 - 10.1016/j.jas.2013.11.014

DO - 10.1016/j.jas.2013.11.014

M3 - Article

AN - SCOPUS:84890391320

VL - 42

SP - 381

EP - 389

JO - Journal of Archaeological Science

JF - Journal of Archaeological Science

SN - 0305-4403

IS - 1

ER -