Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos

Bharat Thyagarajan, Annie Green Howard, Ramon Durazo-Arvizu, John H. Eckfeldt, Marc Gellman, Ryung S. Kim, Kiang Liu, Armando J Mendez, Frank J. Penedo, Gregory A. Talavera, Marston E. Youngblood, Lihui Zhao, Daniela Sotres-Alvarez

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalClinica Chimica Acta
Volume463
DOIs
StatePublished - Dec 1 2016

Fingerprint

Biomarkers
Hispanic Americans
Health
Individuality
Glucose
Fasting
Age Groups
Blood
Population
Cystatin C
Aptitude
Ferritins
Glucose Tolerance Test
Platelets
Platelet Count
Eosinophils
Sex Characteristics
Hemoglobins
Triglycerides
Neutrophils

Keywords

  • Analytical variation
  • Biomarker variability
  • Hispanics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Thyagarajan, B., Howard, A. G., Durazo-Arvizu, R., Eckfeldt, J. H., Gellman, M., Kim, R. S., ... Sotres-Alvarez, D. (2016). Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos. Clinica Chimica Acta, 463, 129-137. https://doi.org/10.1016/j.cca.2016.10.019

Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos. / Thyagarajan, Bharat; Howard, Annie Green; Durazo-Arvizu, Ramon; Eckfeldt, John H.; Gellman, Marc; Kim, Ryung S.; Liu, Kiang; Mendez, Armando J; Penedo, Frank J.; Talavera, Gregory A.; Youngblood, Marston E.; Zhao, Lihui; Sotres-Alvarez, Daniela.

In: Clinica Chimica Acta, Vol. 463, 01.12.2016, p. 129-137.

Research output: Contribution to journalArticle

Thyagarajan, B, Howard, AG, Durazo-Arvizu, R, Eckfeldt, JH, Gellman, M, Kim, RS, Liu, K, Mendez, AJ, Penedo, FJ, Talavera, GA, Youngblood, ME, Zhao, L & Sotres-Alvarez, D 2016, 'Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos', Clinica Chimica Acta, vol. 463, pp. 129-137. https://doi.org/10.1016/j.cca.2016.10.019
Thyagarajan, Bharat ; Howard, Annie Green ; Durazo-Arvizu, Ramon ; Eckfeldt, John H. ; Gellman, Marc ; Kim, Ryung S. ; Liu, Kiang ; Mendez, Armando J ; Penedo, Frank J. ; Talavera, Gregory A. ; Youngblood, Marston E. ; Zhao, Lihui ; Sotres-Alvarez, Daniela. / Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos. In: Clinica Chimica Acta. 2016 ; Vol. 463. pp. 129-137.
@article{c34d1309e6c44df583afba2fe4c7bd90,
title = "Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos",
abstract = "Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, {\%} eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.",
keywords = "Analytical variation, Biomarker variability, Hispanics",
author = "Bharat Thyagarajan and Howard, {Annie Green} and Ramon Durazo-Arvizu and Eckfeldt, {John H.} and Marc Gellman and Kim, {Ryung S.} and Kiang Liu and Mendez, {Armando J} and Penedo, {Frank J.} and Talavera, {Gregory A.} and Youngblood, {Marston E.} and Lihui Zhao and Daniela Sotres-Alvarez",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.cca.2016.10.019",
language = "English (US)",
volume = "463",
pages = "129--137",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",

}

TY - JOUR

T1 - Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos

AU - Thyagarajan, Bharat

AU - Howard, Annie Green

AU - Durazo-Arvizu, Ramon

AU - Eckfeldt, John H.

AU - Gellman, Marc

AU - Kim, Ryung S.

AU - Liu, Kiang

AU - Mendez, Armando J

AU - Penedo, Frank J.

AU - Talavera, Gregory A.

AU - Youngblood, Marston E.

AU - Zhao, Lihui

AU - Sotres-Alvarez, Daniela

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.

AB - Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.

KW - Analytical variation

KW - Biomarker variability

KW - Hispanics

UR - http://www.scopus.com/inward/record.url?scp=84994013944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994013944&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2016.10.019

DO - 10.1016/j.cca.2016.10.019

M3 - Article

C2 - 27756543

AN - SCOPUS:84994013944

VL - 463

SP - 129

EP - 137

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -