@article{893aad2f24dc46f6a9afade37b25276d,
title = "Analysis of transfer RNA during the early embryogenesis of the freshwater teleost, Heteropneustes fossilis",
abstract = "Total RNA as well as transfer RNA were quantified from mature ova apart from four different embryonic stages namely mid-cleavage, early gastrula, mid-gastrula and organogenesis of the freshwater teleost Heteropneustes fossilis. Total RNA as well as transfer RNA quantity follow a similar variation pattern, being maximum during mid-gastrulation. When analysed by total amino acid acceptance capacity, transfer RNA shows its maximum activity during mid-gastrulation. This coincides with the higher ratio of tRNA to total RNA at this stage. The relative aminoacylation capacity for Ser, Gly, Asn and Thr are found to be higher (9-34%) compared to that for other amino acids. Total tRNA, resolved into three peaks upon HPLC fractionation, shows a high cumulative peak area during mid-gastrulation and organogenesis. These results indicate a switch over of maternal to embryonic translation machinery during gastrulation.",
keywords = "aminoacyl-tRNA synthetase, embryogenesis, HPLC, total RNA, transfer RNA",
author = "Subbayan Pochi and Malancha Sarkar and Manjula Vinayak",
year = "1995",
month = jun
day = "1",
doi = "10.1007/BF00986501",
language = "English",
volume = "21",
pages = "113--118",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "2",
}