Analysis of transfer RNA during the early embryogenesis of the freshwater teleost, Heteropneustes fossilis

P. R. Subbarayan, Malancha Sarkar, Manjula Vinayak

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Total RNA as well as transfer RNA were quantified from mature ova apart from four different embryonic stages namely mid-cleavage, early gastrula, mid-gastrula and organogenesis of the freshwater teleost Heteropneustes fossilis. Total RNA as well as transfer RNA quantity follow a similar variation pattern, being maximum during mid-gastrulation. When analysed by total amino acid acceptance capacity, transfer RNA shows its maximum activity during mid-gastrulation. This coincides with the higher ratio of tRNA to total RNA at this stage. The relative aminoacylation capacity for Ser, Gly, Asn and Thr are found to be higher (9-34%) compared to that for other amino acids. Total tRNA, resolved into three peaks upon HPLC fractionation, shows a high cumulative peak area during mid-gastrulation and organogenesis. These results indicate a switch over of maternal to embryonic translation machinery during gastrulation.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalMolecular Biology Reports
Volume21
Issue number2
DOIs
StatePublished - Jun 1 1995

Keywords

  • aminoacyl-tRNA synthetase
  • embryogenesis
  • HPLC
  • total RNA
  • transfer RNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

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