Analysis of progenitor cell-scaffold combinations by in vivo non-invasive photonic imaging

Irene Román, Marta Vilalta, Julio Rodriguez, Annette M. Matthies, Samer Srouji, Erella Livne, Jeffrey A. Hubbell, Nuria Rubio, Jerónimo Blanco

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Recent developments in stem cell research have promoted a flourishing of new biomaterials and scaffolds for tissue repair. However, there is a scarcity of procedures to monitor the performance of scaffold-stem cell combinations implanted in live animals, avoiding the inherent artefacts associated with in vitro assay conditions. We report the implementation of a procedure based on the use of the luciferase gene as a cell proliferation tracer to monitor, by in vivo non-invasive imaging, the performance of stem cell-biomaterial combinations used for tissue regeneration. In a model system using immunodepressed mice we show preference of a mouse embryonic mesenchymal cell line (C3H/10T1/2) for specific implantation sites and biomaterials during a prolonged in vivo growth period (3 months). Moreover, we analyzed the safety of implanted cells using a sensitive luminometric procedure and showed that the implanted cells did not spread to other organs. Our results demonstrate the utility of this simple and resource-saving procedure in the development and screening of biomaterials for tissue engineering.

Original languageEnglish (US)
Pages (from-to)2718-2728
Number of pages11
JournalBiomaterials
Volume28
Issue number17
DOIs
StatePublished - Jun 1 2007

Keywords

  • Cell proliferation
  • Enhanced green fluorescent protein (EGFP)
  • In vivo imaging
  • Luciferase
  • Scaffold

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Analysis of progenitor cell-scaffold combinations by in vivo non-invasive photonic imaging'. Together they form a unique fingerprint.

  • Cite this

    Román, I., Vilalta, M., Rodriguez, J., Matthies, A. M., Srouji, S., Livne, E., Hubbell, J. A., Rubio, N., & Blanco, J. (2007). Analysis of progenitor cell-scaffold combinations by in vivo non-invasive photonic imaging. Biomaterials, 28(17), 2718-2728. https://doi.org/10.1016/j.biomaterials.2007.02.011