Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms

G. Tsechpenakis, J. Eugenin, J. G. Nicholls, Kenneth J Muller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Optical recording of the activity of hundreds of individual neurons simultaneously within the functioning brain is now possible with calcium sensitive dyes. This offers a major advance over the limitations of single-unit recording with arrays of microelectrodes, or with functional MRI. However, the analysis of optical activity to understand neuronal interactions and circuitry underlying physiological functions requires new computational approaches. Recently it has been possible to record optically from the distributed population of neurons in the brain stem generating the respiratory rhythm, breath by breath, using the compact brain stem and spinal cord preparation of the fetal mouse stained in vitro with calcium-sensitive dye. The simultaneous electrical activity of phrenic motoneurons that innervate the diaphragm measures the timing of inspiratory breaths. In the present work, fluorescence micrographs taken at 4-100 Hz over 20-40 sec have been analyzed with the simultaneously recorded electrical signal from the phrenic nerve, in a Conditional Random Field framework. This computational analysis will be a useful tool for understanding the cellular circuitry in the living brain controlling fundamental physiological processes.

Original languageEnglish
Title of host publicationProceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
Pages1251-1254
Number of pages4
DOIs
StatePublished - Nov 17 2009
Event2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 - Boston, MA, United States
Duration: Jun 28 2009Jul 1 2009

Other

Other2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
CountryUnited States
CityBoston, MA
Period6/28/097/1/09

Fingerprint

Optical Rotation
Diaphragm
Brain Stem
Brain
Coloring Agents
Physiological Phenomena
Calcium
Neurons
Phrenic Nerve
Microelectrodes
Motor Neurons
Spinal Cord
Dyes
Fluorescence
Magnetic Resonance Imaging
Optical recording
Diaphragms
Population
In Vitro Techniques

Keywords

  • 2-photon microscopy
  • Brainstem cell activity
  • Calcium imaging
  • Conditional random fields
  • Respiratory rhythms

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Tsechpenakis, G., Eugenin, J., Nicholls, J. G., & Muller, K. J. (2009). Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. In Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 (pp. 1251-1254). [5193289] https://doi.org/10.1109/ISBI.2009.5193289

Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. / Tsechpenakis, G.; Eugenin, J.; Nicholls, J. G.; Muller, Kenneth J.

Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009. 2009. p. 1251-1254 5193289.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsechpenakis, G, Eugenin, J, Nicholls, JG & Muller, KJ 2009, Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. in Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009., 5193289, pp. 1251-1254, 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009, Boston, MA, United States, 6/28/09. https://doi.org/10.1109/ISBI.2009.5193289
Tsechpenakis G, Eugenin J, Nicholls JG, Muller KJ. Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. In Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009. 2009. p. 1251-1254. 5193289 https://doi.org/10.1109/ISBI.2009.5193289
Tsechpenakis, G. ; Eugenin, J. ; Nicholls, J. G. ; Muller, Kenneth J. / Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009. 2009. pp. 1251-1254
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