Analysis of myelinating Schwann cells in human skin biopsies

Mario da Cunha Saporta, Renata de Moraes Maciel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The human skin is richly innervated by nerve fibers of different calibers and functions, including thickly myelinated large fibers that act as afferents for mechanoreceptors in the dermal papillae. Skin biopsies offer minimally invasive access to these myelinated fibers, in which each internode represents an individual myelinating Schwann cell. Using this approach, human myelinated nerve fibers can be analyzed by several methods, including immunostaining, morphometric and ultrastructural analysis, and molecular biology techniques. This analysis can reveal important aspects of human Schwann cell biology in health and disease, such as in the case of demyelinating neuropathies. This technique has revealed Schwann cell phenotypes in Charcot-Marie-Tooth disease type 1 and acquired inflammatory neuropathies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages359-369
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Keywords

  • Charcot-Marie-Tooth disease
  • Immunohistochemistry
  • Myelinated nerve fibers
  • Neuropathy
  • Schwann cells
  • Skin biopsy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    da Cunha Saporta, M., & de Moraes Maciel, R. (2018). Analysis of myelinating Schwann cells in human skin biopsies. In Methods in Molecular Biology (pp. 359-369). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_24