Analysis of myelinating Schwann cells in human skin biopsies

Mario da Cunha Saporta, Renata de Moraes Maciel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The human skin is richly innervated by nerve fibers of different calibers and functions, including thickly myelinated large fibers that act as afferents for mechanoreceptors in the dermal papillae. Skin biopsies offer minimally invasive access to these myelinated fibers, in which each internode represents an individual myelinating Schwann cell. Using this approach, human myelinated nerve fibers can be analyzed by several methods, including immunostaining, morphometric and ultrastructural analysis, and molecular biology techniques. This analysis can reveal important aspects of human Schwann cell biology in health and disease, such as in the case of demyelinating neuropathies. This technique has revealed Schwann cell phenotypes in Charcot-Marie-Tooth disease type 1 and acquired inflammatory neuropathies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages359-369
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Fingerprint

Schwann Cells
Biopsy
Skin
Charcot-Marie-Tooth Disease
Myelinated Nerve Fibers
Mechanoreceptors
Nerve Fibers
Cell Biology
Molecular Biology
Phenotype
Health

Keywords

  • Charcot-Marie-Tooth disease
  • Immunohistochemistry
  • Myelinated nerve fibers
  • Neuropathy
  • Schwann cells
  • Skin biopsy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

da Cunha Saporta, M., & de Moraes Maciel, R. (2018). Analysis of myelinating Schwann cells in human skin biopsies. In Methods in Molecular Biology (pp. 359-369). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_24

Analysis of myelinating Schwann cells in human skin biopsies. / da Cunha Saporta, Mario; de Moraes Maciel, Renata.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 359-369 (Methods in Molecular Biology; Vol. 1739).

Research output: Chapter in Book/Report/Conference proceedingChapter

da Cunha Saporta, M & de Moraes Maciel, R 2018, Analysis of myelinating Schwann cells in human skin biopsies. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1739, Humana Press Inc., pp. 359-369. https://doi.org/10.1007/978-1-4939-7649-2_24
da Cunha Saporta M, de Moraes Maciel R. Analysis of myelinating Schwann cells in human skin biopsies. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 359-369. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7649-2_24
da Cunha Saporta, Mario ; de Moraes Maciel, Renata. / Analysis of myelinating Schwann cells in human skin biopsies. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 359-369 (Methods in Molecular Biology).
@inbook{811441a47c3e4e3e8f259d846c293074,
title = "Analysis of myelinating Schwann cells in human skin biopsies",
abstract = "The human skin is richly innervated by nerve fibers of different calibers and functions, including thickly myelinated large fibers that act as afferents for mechanoreceptors in the dermal papillae. Skin biopsies offer minimally invasive access to these myelinated fibers, in which each internode represents an individual myelinating Schwann cell. Using this approach, human myelinated nerve fibers can be analyzed by several methods, including immunostaining, morphometric and ultrastructural analysis, and molecular biology techniques. This analysis can reveal important aspects of human Schwann cell biology in health and disease, such as in the case of demyelinating neuropathies. This technique has revealed Schwann cell phenotypes in Charcot-Marie-Tooth disease type 1 and acquired inflammatory neuropathies.",
keywords = "Charcot-Marie-Tooth disease, Immunohistochemistry, Myelinated nerve fibers, Neuropathy, Schwann cells, Skin biopsy",
author = "{da Cunha Saporta}, Mario and {de Moraes Maciel}, Renata",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7649-2_24",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "359--369",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Analysis of myelinating Schwann cells in human skin biopsies

AU - da Cunha Saporta, Mario

AU - de Moraes Maciel, Renata

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The human skin is richly innervated by nerve fibers of different calibers and functions, including thickly myelinated large fibers that act as afferents for mechanoreceptors in the dermal papillae. Skin biopsies offer minimally invasive access to these myelinated fibers, in which each internode represents an individual myelinating Schwann cell. Using this approach, human myelinated nerve fibers can be analyzed by several methods, including immunostaining, morphometric and ultrastructural analysis, and molecular biology techniques. This analysis can reveal important aspects of human Schwann cell biology in health and disease, such as in the case of demyelinating neuropathies. This technique has revealed Schwann cell phenotypes in Charcot-Marie-Tooth disease type 1 and acquired inflammatory neuropathies.

AB - The human skin is richly innervated by nerve fibers of different calibers and functions, including thickly myelinated large fibers that act as afferents for mechanoreceptors in the dermal papillae. Skin biopsies offer minimally invasive access to these myelinated fibers, in which each internode represents an individual myelinating Schwann cell. Using this approach, human myelinated nerve fibers can be analyzed by several methods, including immunostaining, morphometric and ultrastructural analysis, and molecular biology techniques. This analysis can reveal important aspects of human Schwann cell biology in health and disease, such as in the case of demyelinating neuropathies. This technique has revealed Schwann cell phenotypes in Charcot-Marie-Tooth disease type 1 and acquired inflammatory neuropathies.

KW - Charcot-Marie-Tooth disease

KW - Immunohistochemistry

KW - Myelinated nerve fibers

KW - Neuropathy

KW - Schwann cells

KW - Skin biopsy

UR - http://www.scopus.com/inward/record.url?scp=85044219691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044219691&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7649-2_24

DO - 10.1007/978-1-4939-7649-2_24

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 359

EP - 369

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -