Analysis of insulin-like growth factor i gene expression in malignancy: Evidence for a paracrine role in human breast cancer

Douglas Yee, Soonmyoung Paik, Gail S. Lebovic, Rachel R. Marcus, Roberto E. Favoni, Kevin J. Cullen, Marc E. Lippman, Neal Rosen

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Abstract

Insulin-like growth factor I (IGF-I) activity has been reported to be produced by several human cancers. Identification of RNAs transcribed from the IGF-I gene has been complicated by the detection of multiple hybridizing bands on Northern analysis. To determine if any of these RNAs are transcribed from the IGF-I gene, we have used a sensitive and specific ribonuclease (RNAse) protection assay for IGF-I. We have also studied the breast cancer tissue expression of IGF-I using in situ hybridization histochemistry. We have found no IGF-I mRNA in breast (zero of 11) or colon cancer (zero of 9) cell lines; both of these tumors have been previously reported to express IGF-I mRNA. However, three of three neuroepithelioma and one of two Ewing’s sarcoma cell lines express IGF-I mRNA; therefore, in these tumors IGF-I may be an autocrine growth factor. In contrast to breast cancer cell lines, RNA extracted from breast tissues has easily detectable IGF-I mRNA. In situ hybridizations show that IGF-I mRNA is expressed in the stromal cells, and not by normal or malignant epithelial cells. These findings suggest that although IGF-I is not produced by breast epithelial cells it may function as either a paracrine stimulator of epithelial cells or an autocrine stimulator of stromal cells.

Original languageEnglish (US)
Pages (from-to)509-517
Number of pages9
JournalMolecular Endocrinology
Volume3
Issue number3
DOIs
StatePublished - Mar 1989

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ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Yee, D., Paik, S., Lebovic, G. S., Marcus, R. R., Favoni, R. E., Cullen, K. J., Lippman, M. E., & Rosen, N. (1989). Analysis of insulin-like growth factor i gene expression in malignancy: Evidence for a paracrine role in human breast cancer. Molecular Endocrinology, 3(3), 509-517. https://doi.org/10.1210/mend-3-3-509