Analysis of endogenous protein interactions of polycomb group of proteins in mouse embryonic stem cells

LLuis Morey, Luciano Di Croce

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

PRC1 complexes contain four core subunits: Pcgf, Phc, Ring1, and Cbx proteins. Interestingly, mammalian genomes have several paralogues for each subunit, which are differentially expressed depending on the cell type, differentiation program, and cellular stimuli. Therefore, identification and characterization of the specific architecture of different PRC1 complexes during cellular differentiation are essential to better understand the function and recruitment mechanism of PRC1 complexes. In this chapter we describe several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells.

Original languageEnglish (US)
Pages (from-to)153-165
Number of pages13
JournalMethods in Molecular Biology
Volume1480
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Keywords

  • Differentiation
  • Immunoprecipitation
  • Pluripotent stem cells
  • Polycomb

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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