Analysis of basic fibroblast growth factor in rats with inherited retinal degeneration

M. J. McLaren; W. An; M. E. Brown; G. Inana

doi:10.1016/0014-5793(96)00461-9

Analysis of basic fibroblast growth factor in rats with inherited retinal degeneration

M. J. McLaren, W. An, M. E. Brown, G. Inana

Ophthalmology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

In RCS rats, photoreceptors degenerate between postnatal days 20 and 60, secondary to a genetic defect expressed in the neonatal retinal pigmented epithelium (RPE). Previous work has shown delay of the photoreceptor degeneration in this model by intraocular injection of basic fibroblast growth factor bFGF). Evidence is presented here, from bFGF immunostaining and Northern analysis of bFGF mRNA, for reduced bFGF expression in uncultured RPE of dystrophic RCS pups. It is also shown that in the mutant eyes angiogenesis in the underlying choroid, which normally occurs between postnatal days 7 and 10, is markedly delayed, with irregular distribution of vessels, consistent with a reduction in this known angiogenesis factor. Mutational analysis of the bFGF transcript and gene by denaturing gradient gel electrophoresis and Southern analysis did not, however, reveal abnormalities in the coding sequence of this gene in RCS rats.

Original language	English (US)
Pages (from-to)	63-70
Number of pages	8
Journal	FEBS letters
Volume	387
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(96)00461-9
State	Published - May 27 1996

Keywords

Angiogenesis
Basic FGF
Choroid
Mutational analysis
Pigment epithelium of eye
RCS rat

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Analysis of basic fibroblast growth factor in rats with inherited retinal degeneration",

abstract = "In RCS rats, photoreceptors degenerate between postnatal days 20 and 60, secondary to a genetic defect expressed in the neonatal retinal pigmented epithelium (RPE). Previous work has shown delay of the photoreceptor degeneration in this model by intraocular injection of basic fibroblast growth factor bFGF). Evidence is presented here, from bFGF immunostaining and Northern analysis of bFGF mRNA, for reduced bFGF expression in uncultured RPE of dystrophic RCS pups. It is also shown that in the mutant eyes angiogenesis in the underlying choroid, which normally occurs between postnatal days 7 and 10, is markedly delayed, with irregular distribution of vessels, consistent with a reduction in this known angiogenesis factor. Mutational analysis of the bFGF transcript and gene by denaturing gradient gel electrophoresis and Southern analysis did not, however, reveal abnormalities in the coding sequence of this gene in RCS rats.",

keywords = "Angiogenesis, Basic FGF, Choroid, Mutational analysis, Pigment epithelium of eye, RCS rat",

author = "McLaren, {M. J.} and W. An and Brown, {M. E.} and G. Inana",

note = "Funding Information: Acknowledgements: The authors wish to acknowledge the excellent technical support provided by Helena Lau and Magda Celdran (histology) and Barbara French (photography). This work was supported in part by Research to Prevent Blindness (RPB), Inc. including a Career Development Award to M.J.M., by Walter G. Ross Foundation and Foundation Fighting Blindness.",

year = "1996",

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doi = "10.1016/0014-5793(96)00461-9",

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AU - An, W.

AU - Brown, M. E.

AU - Inana, G.

N1 - Funding Information: Acknowledgements: The authors wish to acknowledge the excellent technical support provided by Helena Lau and Magda Celdran (histology) and Barbara French (photography). This work was supported in part by Research to Prevent Blindness (RPB), Inc. including a Career Development Award to M.J.M., by Walter G. Ross Foundation and Foundation Fighting Blindness.

PY - 1996/5/27

Y1 - 1996/5/27

N2 - In RCS rats, photoreceptors degenerate between postnatal days 20 and 60, secondary to a genetic defect expressed in the neonatal retinal pigmented epithelium (RPE). Previous work has shown delay of the photoreceptor degeneration in this model by intraocular injection of basic fibroblast growth factor bFGF). Evidence is presented here, from bFGF immunostaining and Northern analysis of bFGF mRNA, for reduced bFGF expression in uncultured RPE of dystrophic RCS pups. It is also shown that in the mutant eyes angiogenesis in the underlying choroid, which normally occurs between postnatal days 7 and 10, is markedly delayed, with irregular distribution of vessels, consistent with a reduction in this known angiogenesis factor. Mutational analysis of the bFGF transcript and gene by denaturing gradient gel electrophoresis and Southern analysis did not, however, reveal abnormalities in the coding sequence of this gene in RCS rats.

AB - In RCS rats, photoreceptors degenerate between postnatal days 20 and 60, secondary to a genetic defect expressed in the neonatal retinal pigmented epithelium (RPE). Previous work has shown delay of the photoreceptor degeneration in this model by intraocular injection of basic fibroblast growth factor bFGF). Evidence is presented here, from bFGF immunostaining and Northern analysis of bFGF mRNA, for reduced bFGF expression in uncultured RPE of dystrophic RCS pups. It is also shown that in the mutant eyes angiogenesis in the underlying choroid, which normally occurs between postnatal days 7 and 10, is markedly delayed, with irregular distribution of vessels, consistent with a reduction in this known angiogenesis factor. Mutational analysis of the bFGF transcript and gene by denaturing gradient gel electrophoresis and Southern analysis did not, however, reveal abnormalities in the coding sequence of this gene in RCS rats.

KW - Angiogenesis

KW - Basic FGF

KW - Choroid

KW - Mutational analysis

KW - Pigment epithelium of eye

KW - RCS rat

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Analysis of basic fibroblast growth factor in rats with inherited retinal degeneration

Abstract

Keywords

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