Analyses of cholesterol metabolites of optic nerve using GC-MS methods

Gurkaran S. Sarohia, Erik D. Garza, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-51
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cholesterol
  • Gas chromatography-mass spectrometry (GC-MS)
  • Lipid extraction
  • Lipidomics
  • Lipids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Sarohia, G. S., Garza, E. D., & Bhattacharya, S. K. (2019). Analyses of cholesterol metabolites of optic nerve using GC-MS methods. In Methods in Molecular Biology (pp. 47-51). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_5