Analyses of cholesterol metabolites of optic nerve using GC-MS methods

Gurkaran S. Sarohia, Erik D. Garza, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-51
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745

Fingerprint

Optic Nerve
Gas Chromatography-Mass Spectrometry
Cholesterol
Lipids
Nerve Tissue
Nerve Agents

Keywords

  • Cholesterol
  • Gas chromatography-mass spectrometry (GC-MS)
  • Lipid extraction
  • Lipidomics
  • Lipids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sarohia, G. S., Garza, E. D., & Bhattacharya, S. K. (2019). Analyses of cholesterol metabolites of optic nerve using GC-MS methods. In Methods in Molecular Biology (pp. 47-51). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_5

Analyses of cholesterol metabolites of optic nerve using GC-MS methods. / Sarohia, Gurkaran S.; Garza, Erik D.; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 47-51 (Methods in Molecular Biology; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sarohia, GS, Garza, ED & Bhattacharya, SK 2019, Analyses of cholesterol metabolites of optic nerve using GC-MS methods. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1996, Humana Press Inc., pp. 47-51. https://doi.org/10.1007/978-1-4939-9488-5_5
Sarohia GS, Garza ED, Bhattacharya SK. Analyses of cholesterol metabolites of optic nerve using GC-MS methods. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 47-51. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9488-5_5
Sarohia, Gurkaran S. ; Garza, Erik D. ; Bhattacharya, Sanjoy K. / Analyses of cholesterol metabolites of optic nerve using GC-MS methods. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 47-51 (Methods in Molecular Biology).
@inbook{1fb5f7a574054312bc869540a79614e3,
title = "Analyses of cholesterol metabolites of optic nerve using GC-MS methods",
abstract = "Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.",
keywords = "Cholesterol, Gas chromatography-mass spectrometry (GC-MS), Lipid extraction, Lipidomics, Lipids",
author = "Sarohia, {Gurkaran S.} and Garza, {Erik D.} and Bhattacharya, {Sanjoy K}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9488-5_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "47--51",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Analyses of cholesterol metabolites of optic nerve using GC-MS methods

AU - Sarohia, Gurkaran S.

AU - Garza, Erik D.

AU - Bhattacharya, Sanjoy K

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.

AB - Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.

KW - Cholesterol

KW - Gas chromatography-mass spectrometry (GC-MS)

KW - Lipid extraction

KW - Lipidomics

KW - Lipids

UR - http://www.scopus.com/inward/record.url?scp=85066252967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066252967&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9488-5_5

DO - 10.1007/978-1-4939-9488-5_5

M3 - Chapter

C2 - 31127546

AN - SCOPUS:85066252967

T3 - Methods in Molecular Biology

SP - 47

EP - 51

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -