Analogs of growth hormone-releasing hormone induce release of growth hormone in the bovine.

R. Scarborough, J. Gulyas, Andrew V Schally, J. J. Reeves

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Biological potencies of three 29 amino acid growth hormone-releasing hormone analogs (GHRH[1-29]) were determined in the bovine and compared to synthetic human GHRH (44 amino acids; hGHRH[1-44]NH2) for their ability to increase serum growth hormone (GH) concentrations. Four prepubertal Holstein heifers (179 +/- 10 kg) received hGHRH(1-44)NH2 or analogs (D-Ala2, Nle27, Agm29 GHRH[1-29], [JG-73]; D-N-MeAla2, Nle27, Agm29 GHRH[1-29], [JG-75]; and desamino-Tyr1, D-Ala2, Nle27, Agm29 GHRH[1-29], [JG-77]) at the following doses: 0, 6.25, 25, 100 and 400 micrograms/animal. All treatment-dose combinations were administered to each heifer with at least a 1-d interval between treatments. Sixteen blood samples were collected via jugular cannulas 20 min before and up to 6 h after treatment injection. There was a linear dose-dependent GH release in response to hGHRH(1-44)NH2 and the three analogs. Growth hormone peak amplitudes for the three analogs were similar to those observed after administration of the hGHRH(1-44)NH2 (P greater than .05). However, when total area under the GH response curves for each treatment was averaged over all the doses, JG-73 stimulated greater GH release than hGHRH(1-44)NH2 (P less than .05) Heifers injected with the 400-microgram dose of hGHRH(1-44)NH2 or the three analogs showed a primary release of GH followed by a secondary release 1 h later. At all other doses, only a primary GH release was observed.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)1386-1392
Number of pages7
JournalJournal of Animal Science
Volume66
Issue number6
StatePublished - Jun 1 1988
Externally publishedYes

Fingerprint

somatoliberin
Growth Hormone-Releasing Hormone
somatotropin
Growth Hormone
dosage
heifers
Amino Acids
synthetic hormones
amino acids
Therapeutics
cannulas
blood serum
bovine growth hormone
neck
Neck
Holstein
injection
Injections
somatotropin releasing hormone (1-29)
cattle

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Analogs of growth hormone-releasing hormone induce release of growth hormone in the bovine. / Scarborough, R.; Gulyas, J.; Schally, Andrew V; Reeves, J. J.

In: Journal of Animal Science, Vol. 66, No. 6, 01.06.1988, p. 1386-1392.

Research output: Contribution to journalArticle

Scarborough, R. ; Gulyas, J. ; Schally, Andrew V ; Reeves, J. J. / Analogs of growth hormone-releasing hormone induce release of growth hormone in the bovine. In: Journal of Animal Science. 1988 ; Vol. 66, No. 6. pp. 1386-1392.
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abstract = "Biological potencies of three 29 amino acid growth hormone-releasing hormone analogs (GHRH[1-29]) were determined in the bovine and compared to synthetic human GHRH (44 amino acids; hGHRH[1-44]NH2) for their ability to increase serum growth hormone (GH) concentrations. Four prepubertal Holstein heifers (179 +/- 10 kg) received hGHRH(1-44)NH2 or analogs (D-Ala2, Nle27, Agm29 GHRH[1-29], [JG-73]; D-N-MeAla2, Nle27, Agm29 GHRH[1-29], [JG-75]; and desamino-Tyr1, D-Ala2, Nle27, Agm29 GHRH[1-29], [JG-77]) at the following doses: 0, 6.25, 25, 100 and 400 micrograms/animal. All treatment-dose combinations were administered to each heifer with at least a 1-d interval between treatments. Sixteen blood samples were collected via jugular cannulas 20 min before and up to 6 h after treatment injection. There was a linear dose-dependent GH release in response to hGHRH(1-44)NH2 and the three analogs. Growth hormone peak amplitudes for the three analogs were similar to those observed after administration of the hGHRH(1-44)NH2 (P greater than .05). However, when total area under the GH response curves for each treatment was averaged over all the doses, JG-73 stimulated greater GH release than hGHRH(1-44)NH2 (P less than .05) Heifers injected with the 400-microgram dose of hGHRH(1-44)NH2 or the three analogs showed a primary release of GH followed by a secondary release 1 h later. At all other doses, only a primary GH release was observed.(ABSTRACT TRUNCATED AT 250 WORDS)",
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