An unusual gene arrangement for the putative chromosome replication origin and circadian expression of dnaN in Synechococcus sp. strain PCC 7942

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Abstract

In eubacteria, the clustering of DnaA boxes around the dnaN (β subunit of DNA polymerase III) and dnaA genes usually defines the chromosome replication origin (oriC). In this study, the dnaN locus from the cyanobacterium Synechococcus sp. strain PCC 7942 was sequenced. The gene order in this region is cbbZp-dnaN-orf288-purL-purF which contrasts with other eubacteria. A cluster of eleven DnaA boxes (consensus sequence: TTTTCCACA) was found in the intergenic region between dnaN and cbbZp. We also found a 41-bp sequence within this region that is 80% identical to the proposed oriC of Streptomyces coelicolor. Therefore, we propose that this intergenic region may serve as an oriC in Synechococcus. Using bacterial luciferase as a reporter, we also showed that dnaN is rhythmically expressed, suggesting that DNA replication could be under circadian control in this organism.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalGene
Volume172
Issue number1
DOIs
StatePublished - Jun 12 1996
Externally publishedYes

Fingerprint

Synechococcus
Intergenic DNA
Replication Origin
Gene Order
Bacterial Luciferases
Chromosomes
DNA Polymerase III
Streptomyces coelicolor
Bacteria
Consensus Sequence
Cyanobacteria
DNA Replication
Cluster Analysis
Genes

Keywords

  • Circadian rhythm
  • Cyanobacteria
  • DnaA boxes
  • Luciferase
  • luxAB
  • oriC

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "An unusual gene arrangement for the putative chromosome replication origin and circadian expression of dnaN in Synechococcus sp. strain PCC 7942",
abstract = "In eubacteria, the clustering of DnaA boxes around the dnaN (β subunit of DNA polymerase III) and dnaA genes usually defines the chromosome replication origin (oriC). In this study, the dnaN locus from the cyanobacterium Synechococcus sp. strain PCC 7942 was sequenced. The gene order in this region is cbbZp-dnaN-orf288-purL-purF which contrasts with other eubacteria. A cluster of eleven DnaA boxes (consensus sequence: TTTTCCACA) was found in the intergenic region between dnaN and cbbZp. We also found a 41-bp sequence within this region that is 80{\%} identical to the proposed oriC of Streptomyces coelicolor. Therefore, we propose that this intergenic region may serve as an oriC in Synechococcus. Using bacterial luciferase as a reporter, we also showed that dnaN is rhythmically expressed, suggesting that DNA replication could be under circadian control in this organism.",
keywords = "Circadian rhythm, Cyanobacteria, DnaA boxes, Luciferase, luxAB, oriC",
author = "Yi Liu and Nicholas Tsinoremas",
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AU - Liu, Yi

AU - Tsinoremas, Nicholas

PY - 1996/6/12

Y1 - 1996/6/12

N2 - In eubacteria, the clustering of DnaA boxes around the dnaN (β subunit of DNA polymerase III) and dnaA genes usually defines the chromosome replication origin (oriC). In this study, the dnaN locus from the cyanobacterium Synechococcus sp. strain PCC 7942 was sequenced. The gene order in this region is cbbZp-dnaN-orf288-purL-purF which contrasts with other eubacteria. A cluster of eleven DnaA boxes (consensus sequence: TTTTCCACA) was found in the intergenic region between dnaN and cbbZp. We also found a 41-bp sequence within this region that is 80% identical to the proposed oriC of Streptomyces coelicolor. Therefore, we propose that this intergenic region may serve as an oriC in Synechococcus. Using bacterial luciferase as a reporter, we also showed that dnaN is rhythmically expressed, suggesting that DNA replication could be under circadian control in this organism.

AB - In eubacteria, the clustering of DnaA boxes around the dnaN (β subunit of DNA polymerase III) and dnaA genes usually defines the chromosome replication origin (oriC). In this study, the dnaN locus from the cyanobacterium Synechococcus sp. strain PCC 7942 was sequenced. The gene order in this region is cbbZp-dnaN-orf288-purL-purF which contrasts with other eubacteria. A cluster of eleven DnaA boxes (consensus sequence: TTTTCCACA) was found in the intergenic region between dnaN and cbbZp. We also found a 41-bp sequence within this region that is 80% identical to the proposed oriC of Streptomyces coelicolor. Therefore, we propose that this intergenic region may serve as an oriC in Synechococcus. Using bacterial luciferase as a reporter, we also showed that dnaN is rhythmically expressed, suggesting that DNA replication could be under circadian control in this organism.

KW - Circadian rhythm

KW - Cyanobacteria

KW - DnaA boxes

KW - Luciferase

KW - luxAB

KW - oriC

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