An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle

James M. Roberts, Gennaro D'Urso

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

An in vitro assay was developed to study the positive factors that regulate the onset of DNA replication during the mammalian cell cycle. Extracts prepared from cells at defined positions in the cell cycle were used to examine the replication of SV40 DNA in a cell free system. Extracts prepared from S phase cells were ten times more efficient at initiating replication at the SV40 origin than were extracts from G1 cells, whereas elongation rates were similar in G1 and S reactions. At a discrete point in the cell cycle, just before the cell's entry into S, an activity appeared that was required, in conjunction with SV40 T antigen, for site specific initiation at the SV40 origin. This factor had a role in unwinding DNA at the replication origin.

Original languageEnglish (US)
Pages (from-to)1486-1489
Number of pages4
JournalScience
Volume241
Issue number4872
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • General

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