An odorant receptor from the Southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants

Julien Pelletier, David T. Hughes, Charles W Luetje, Walter S. Leal

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Background: Insect odorant receptors (ORs) are heteromers comprised of highly variable odorant-binding subunits associated with one conserved co-receptor. They are potential molecular targets for the development of novel mosquito attractants and repellents. ORs have been identified in the malaria mosquito, Anopheles gambiae, and in the yellow fever mosquito, Aedes aegypti. However, they are still unknown in the Southern house mosquito, Culex quinquefasciatus, which transmits pathogens that cause human diseases throughout the world, including West Nile Virus in the United States. Methodology: We have employed a combination of bioinformatics, molecular cloning and electrophysiology approaches to identify and characterize the response profile of an OR in Cx. quinquefasciatus. First, we have unveiled a large multigenic family of one-hundred-fifty-eight putative ORs in this species, including a subgroup of conserved ORs in three mosquito species. Using the Xenopus oocytes expression system, we have determined the response profile of CquiOR2, an antennaespecific OR, which shares high identity with putative orthologs in Anopheles gambiae (AgamOR2) and Aedes aegypti (AaegOR2). Conclusion: We show that CquiOR2 is highly sensitive to indole, an oviposition attractant for Cx. quinquefasciatus. The response profile of CquiOR2 expressed in Xenopus oocytes resembles that of an olfactory receptor neuron housed in the antennal short blunt-tipped sensilla (A2) of Cx. quinquefasciatus, which are natural detectors for oviposition attractants. This first Culex OR de-orphanized is, therefore, a potential molecular target for screening oviposition attractants.

Original languageEnglish
Article numbere10090
JournalPLoS One
Volume5
Issue number4
DOIs
StatePublished - Sep 10 2010

Fingerprint

oviposition attractants
Odorant Receptors
Oviposition
Culex
Culex quinquefasciatus
Culicidae
Aedes aegypti
Anopheles gambiae
Aedes
Xenopus
Oocytes
oocytes
Olfactory Receptor Neurons
Yellow Fever
Sensilla
West Nile virus
electrophysiology
olfactory receptors
Electrophysiology
Molecular Cloning

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

An odorant receptor from the Southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants. / Pelletier, Julien; Hughes, David T.; Luetje, Charles W; Leal, Walter S.

In: PLoS One, Vol. 5, No. 4, e10090, 10.09.2010.

Research output: Contribution to journalArticle

Pelletier, Julien ; Hughes, David T. ; Luetje, Charles W ; Leal, Walter S. / An odorant receptor from the Southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants. In: PLoS One. 2010 ; Vol. 5, No. 4.
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