An iterative self-refining and self-evaluating approach for protein model quality estimation

Zheng Wang, Jianlin Cheng

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Evaluating or predicting the quality of protein models (i.e., predicted protein tertiary structures) without knowing their native structures is important for selecting and appropriately using protein models. We describe an iterative approach that improves the performances of protein Model Quality Assurance Programs (MQAPs). Given the initial quality scores of a list of models assigned by a MQAP, the method iteratively refines the scores until the ranking of the models does not change. We applied the method to the model quality assessment data generated by 30 MQAPs during the Eighth Critical Assessment of Techniques for Protein Structure Prediction. To various degrees, our method increased the average correlation between predicted and real quality scores of 25 out of 30 MQAPs and reduced the average loss (i.e., the difference between the top ranked model and the best model) for 28 MQAPs. Particularly, for MQAPs with low average correlations (<0.4), the correlation can be increased by several times. Similar experiments conducted on the CASP9 MQAPs also demonstrated the effectiveness of the method. Our method is a hybrid method that combines the original method of a MQAP and the pair-wise comparison clustering method. It can achieve a high accuracy similar to a full pair-wise clustering method, but with much less computation time when evaluating hundreds of models. Furthermore, without knowing native structures, the iterative refining method can evaluate the performance of a MQAP by analyzing its model quality predictions. Published by Wiley-Blackwell.

Original languageEnglish (US)
Pages (from-to)142-151
Number of pages10
JournalProtein Science
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • Iterative algorithm
  • Model ranking
  • Protein model quality assessment
  • Protein structure prediction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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