An inward rectifier K+ current modulates in neuroblastoma cells the tyrosine phosphorylation of the ppl25(FAK) and associated proteins

Role in neuritogenesis

Laura Bianchi, A. Arcangeli, P. Bartolini, G. Mugnai, E. Wauke, M. Olivotto

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The relationships between the integrin-mediated activation of inward rectifyier K+ channels (K(IR)), the phosphorylation of pp125(FAK) and the rescue of neuritogenesis were studied in 41A3 mouse neuroblastoma cells. Neuritogenesis, elicited by adhesion to FN-enriched substrata, was reversibly impaired by pretreating these cells with the tyrosine kinase inhibitor Herbimycin A. This impairment mimicked that operated by Cs+ ions, which selectively inhibited the integrin-mediated activation of K(IR) channels. Various phosphotyrosine containing cellular proteins underwent a marked increase upon cell adhesion to FN-coated dishes. This increase was significantly reduced by Cs+ addition. Immunoprecipitation of pp125(FAK) revealed that the phosphorylation of this kinase and several associated proteins was significantly and reversibly inhibited by Cs+, indicating that integrin-mediated activation of K(IR) channels is a limiting step upstream to the phosphorylation of pp125(FAK) in the commitment to neuritogenesis.

Original languageEnglish
Pages (from-to)823-829
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number3
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Phosphorylation
Neuroblastoma
Integrins
Tyrosine
Chemical activation
Phosphotyrosine
Proteins
Cell adhesion
Immunoprecipitation
Cell Adhesion
Protein-Tyrosine Kinases
Phosphotransferases
Adhesion
Ions

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

An inward rectifier K+ current modulates in neuroblastoma cells the tyrosine phosphorylation of the ppl25(FAK) and associated proteins : Role in neuritogenesis. / Bianchi, Laura; Arcangeli, A.; Bartolini, P.; Mugnai, G.; Wauke, E.; Olivotto, M.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 3, 01.01.1995, p. 823-829.

Research output: Contribution to journalArticle

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