An improved test of neurological dysfunction following transient focal cerebral ischemia in rats

Michael E. Sughrue, J. Mocco, Ricardo J Komotar, Anand Mehra, Anthony L. D'Ambrosio, Bartosz T. Grobelny, David L. Penn, E. Sander Connolly

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The Adhesive Removal (sticky-tape) test is a commonly used test of somatosensory dysfunction following cerebral ischemia in rats. This test requires several days of pre-training prior to surgery, which can be time consuming. We present our results with an improved version of the sticky-tape test. Male Wistar rats were subjected to either sham surgery (n = 4) or right middle cerebral artery occlusion (rMCAo) using an intraluminal filament (n = 9), followed by a 10-day survival period. On post-operative days (POD) 1, 3, 7, and 10 animals underwent both the conventional sticky-tape test (CST) with measurement of the time to remove the stimulus (trs), as well as a modified sticky-tape test (MST), in which a non-removable tape sleeve was placed around the animal's paw. Time spent attending to this stimulus (tas) was recorded. Despite 3 days of pre-training, animals undergoing baseline CST still exhibited marked variability in pre-operative baseline test performance (trs range 1-60 s). In contrast, animals undergoing MST for the first time demonstrated nearly uniformly excellent performance (% tas range 91.5-98.5% of the 30 s testing period). Although, affected (left) limb performance on both CST (6.8-fold increase in trs on POD 1 compared to baseline) and MST (100% decrease in tas on POD 1 compared to baseline) was markedly altered by rMCAo, CST performance declined bilaterally, and no significant differences in the ratio of affected (left) and unaffected (right) limb performance between sham-operated and rMCAo animals were observed at any time point. In contrast, the ratio of left to right performance on the MST was significantly different at all time points (P < 0.01). In conclusion, we present a simple modification of the widely used Adhesive Removal test and provide evidence that this test can accurately assess neurological dysfunction in rodents, not only with minimal pre-training, but also with improved localization of the side of injury.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalJournal of Neuroscience Methods
Volume151
Issue number2
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

Fingerprint

Transient Ischemic Attack
Middle Cerebral Artery Infarction
Extremities
Brain Ischemia
Wistar Rats
Rodentia
Wounds and Injuries

Keywords

  • Brain ischemia
  • Cerebral ischemia
  • Functional testing
  • Neurological deficit
  • Neurological function
  • Outcome
  • Pre-training
  • Rodents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An improved test of neurological dysfunction following transient focal cerebral ischemia in rats. / Sughrue, Michael E.; Mocco, J.; Komotar, Ricardo J; Mehra, Anand; D'Ambrosio, Anthony L.; Grobelny, Bartosz T.; Penn, David L.; Connolly, E. Sander.

In: Journal of Neuroscience Methods, Vol. 151, No. 2, 15.03.2006, p. 83-89.

Research output: Contribution to journalArticle

Sughrue, ME, Mocco, J, Komotar, RJ, Mehra, A, D'Ambrosio, AL, Grobelny, BT, Penn, DL & Connolly, ES 2006, 'An improved test of neurological dysfunction following transient focal cerebral ischemia in rats', Journal of Neuroscience Methods, vol. 151, no. 2, pp. 83-89. https://doi.org/10.1016/j.jneumeth.2005.04.023
Sughrue, Michael E. ; Mocco, J. ; Komotar, Ricardo J ; Mehra, Anand ; D'Ambrosio, Anthony L. ; Grobelny, Bartosz T. ; Penn, David L. ; Connolly, E. Sander. / An improved test of neurological dysfunction following transient focal cerebral ischemia in rats. In: Journal of Neuroscience Methods. 2006 ; Vol. 151, No. 2. pp. 83-89.
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