An improved technique for rapid autoradiography of cells and tissue sections

J. M. Langager, Guy Howard, D. J. Baylink

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have demonstrated a method for autoradiography of cell and tissue preparations which is both rapid and safe. The method utilizes only the primary scintillator, PPO, placed under the final emulsion to facilitate activation of the silver grains in the emulsion. Exposure of the autoradiographs is complete under the conditions described within 4 h at ambient temperature. The method is sensitive to exposure time and to the concentration of added radioisotope. The exclusion of volatile, toxic chemicals from the preparations allows the experiments to be performed without any health hazard to the investigator.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalHistochemistry
Volume75
Issue number4
DOIs
StatePublished - Dec 1 1982
Externally publishedYes

Fingerprint

autoradiography
Autoradiography
Emulsions
emulsions
health hazards
Poisons
cells
radionuclides
toxic substances
Silver
Radioisotopes
silver
exposure duration
ambient temperature
methodology
Research Personnel
Temperature
Health
tissues

ASJC Scopus subject areas

  • Anatomy

Cite this

An improved technique for rapid autoradiography of cells and tissue sections. / Langager, J. M.; Howard, Guy; Baylink, D. J.

In: Histochemistry, Vol. 75, No. 4, 01.12.1982, p. 523-531.

Research output: Contribution to journalArticle

Langager, J. M. ; Howard, Guy ; Baylink, D. J. / An improved technique for rapid autoradiography of cells and tissue sections. In: Histochemistry. 1982 ; Vol. 75, No. 4. pp. 523-531.
@article{4ea7b981f39144e2aba670a395fa093a,
title = "An improved technique for rapid autoradiography of cells and tissue sections",
abstract = "We have demonstrated a method for autoradiography of cell and tissue preparations which is both rapid and safe. The method utilizes only the primary scintillator, PPO, placed under the final emulsion to facilitate activation of the silver grains in the emulsion. Exposure of the autoradiographs is complete under the conditions described within 4 h at ambient temperature. The method is sensitive to exposure time and to the concentration of added radioisotope. The exclusion of volatile, toxic chemicals from the preparations allows the experiments to be performed without any health hazard to the investigator.",
author = "Langager, {J. M.} and Guy Howard and Baylink, {D. J.}",
year = "1982",
month = "12",
day = "1",
doi = "10.1007/BF00640603",
language = "English",
volume = "75",
pages = "523--531",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - An improved technique for rapid autoradiography of cells and tissue sections

AU - Langager, J. M.

AU - Howard, Guy

AU - Baylink, D. J.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - We have demonstrated a method for autoradiography of cell and tissue preparations which is both rapid and safe. The method utilizes only the primary scintillator, PPO, placed under the final emulsion to facilitate activation of the silver grains in the emulsion. Exposure of the autoradiographs is complete under the conditions described within 4 h at ambient temperature. The method is sensitive to exposure time and to the concentration of added radioisotope. The exclusion of volatile, toxic chemicals from the preparations allows the experiments to be performed without any health hazard to the investigator.

AB - We have demonstrated a method for autoradiography of cell and tissue preparations which is both rapid and safe. The method utilizes only the primary scintillator, PPO, placed under the final emulsion to facilitate activation of the silver grains in the emulsion. Exposure of the autoradiographs is complete under the conditions described within 4 h at ambient temperature. The method is sensitive to exposure time and to the concentration of added radioisotope. The exclusion of volatile, toxic chemicals from the preparations allows the experiments to be performed without any health hazard to the investigator.

UR - http://www.scopus.com/inward/record.url?scp=0020352007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020352007&partnerID=8YFLogxK

U2 - 10.1007/BF00640603

DO - 10.1007/BF00640603

M3 - Article

VL - 75

SP - 523

EP - 531

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 4

ER -