A method of using cover slips in establishing human fibroblast cultures from explants is described. This procedure has three important advantages. Firstly, the cover slips with cells may be removed from the explants and transferred without the use of trypsin. Secondly, cells grow out from the explants within 48 hours and ready to be transferred within two weeks. Thirdly, it eliminates the problems of detachment of the explants from culture dishes.
ASJC Scopus subject areas
- Pathology and Forensic Medicine