An important role for protein kinase C-δ in human keratinocyte migration on dermal collagen

Wei Li, Celina Nadelman, Noah S. Gratch, Weiquin Li, Mei Chen, Noriyuki Kasahara, David T. Woodley

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Migration of human keratinocytes plays a critical role in the re-epithelialization of human skin wounds, the process by which the wound bed is resurfaced and closed by keratinocytes as it forms a new epidermis. While the importance of ECM components and serum factors in the regulation of keratinocytes motility is well established, the intracellular signaling mechanisms remain fragmentary. In this study, we investigated the role of protein kinase Cδ (PKCδ) signaling in the promotion of human keratinocyte migration by a collagen matrix and bovine pituitary extract. We found that pharmacological inhibition of the PKCδ pathway completely blocks migration. Using a lentivirus-based vector system, which offers more than 90% gene transduction efficiency to human keratinocytes, we show that the kinase-defective mutant of PKCδ (K376R) dramatically inhibits human keratinocyte migration. Furthermore, PKCδ is activated in migrating human keratinocytes. These observations indicate for the first time that the PKCδ pathway plays an important role in the control of human keratinocyte migration.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalExperimental Cell Research
Volume273
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

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Keratinocytes
Protein Kinase C
Collagen
Skin
Re-Epithelialization
Lentivirus
Wounds and Injuries
Mutant Proteins
Epidermis
Phosphotransferases
Pharmacology
Serum
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

An important role for protein kinase C-δ in human keratinocyte migration on dermal collagen. / Li, Wei; Nadelman, Celina; Gratch, Noah S.; Li, Weiquin; Chen, Mei; Kasahara, Noriyuki; Woodley, David T.

In: Experimental Cell Research, Vol. 273, No. 2, 2002, p. 219-228.

Research output: Contribution to journalArticle

Li, Wei ; Nadelman, Celina ; Gratch, Noah S. ; Li, Weiquin ; Chen, Mei ; Kasahara, Noriyuki ; Woodley, David T. / An important role for protein kinase C-δ in human keratinocyte migration on dermal collagen. In: Experimental Cell Research. 2002 ; Vol. 273, No. 2. pp. 219-228.
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