An immunological factor that affects Anopheles gambiae survival.

B. H. Noden, J. A. Vaughan, M. S. Ibrahim, John C Beier

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

High titers of antibodies against Anopheles gambiae midguts were produced in New Zealand rabbits to identify midgut targets for an antimosquito vaccine. The serum from one of 8 rabbits (designated R2B6) killed 71.6% (Abbott's adjusted % mortality) of An. gambiae within 7 days. Mosquitoes ingesting R2B6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. Anopheles stephensi and Anopheles arabiensis were also killed when ingesting R2B6 serum but Anopheles freeborni, Anopheles albimanus, and Aedes aegypti were not affected. The mosquitocidal factor was a relatively large molecule (> 100,000 MW) maintained at threshold levels in the sera and killing was complement independent. Mortality, however, was not IgG mediated, as determined by protein A-sepharose fractionation. This surprising finding confounds possibilities of using antibodies against whole mosquito midguts as a step in the development of antimosquito vaccines.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalJournal of the American Mosquito Control Association
Volume11
Issue number1
StatePublished - Mar 1 1995
Externally publishedYes

Fingerprint

immunologic factors
Anopheles gambiae
Anopheles
Immunologic Factors
midgut
serum
Culicidae
vaccine
Anopheles freeborni
mosquito
Serum
rabbits
Anopheles arabiensis
Anopheles albimanus
antibody
Anopheles stephensi
antibodies
Vaccines
blood meal
vaccine development

ASJC Scopus subject areas

  • Insect Science

Cite this

An immunological factor that affects Anopheles gambiae survival. / Noden, B. H.; Vaughan, J. A.; Ibrahim, M. S.; Beier, John C.

In: Journal of the American Mosquito Control Association, Vol. 11, No. 1, 01.03.1995, p. 45-49.

Research output: Contribution to journalArticle

Noden, B. H. ; Vaughan, J. A. ; Ibrahim, M. S. ; Beier, John C. / An immunological factor that affects Anopheles gambiae survival. In: Journal of the American Mosquito Control Association. 1995 ; Vol. 11, No. 1. pp. 45-49.
@article{ffc58ea0ed3a4d7ba5c3ac0cdbb60897,
title = "An immunological factor that affects Anopheles gambiae survival.",
abstract = "High titers of antibodies against Anopheles gambiae midguts were produced in New Zealand rabbits to identify midgut targets for an antimosquito vaccine. The serum from one of 8 rabbits (designated R2B6) killed 71.6{\%} (Abbott's adjusted {\%} mortality) of An. gambiae within 7 days. Mosquitoes ingesting R2B6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. Anopheles stephensi and Anopheles arabiensis were also killed when ingesting R2B6 serum but Anopheles freeborni, Anopheles albimanus, and Aedes aegypti were not affected. The mosquitocidal factor was a relatively large molecule (> 100,000 MW) maintained at threshold levels in the sera and killing was complement independent. Mortality, however, was not IgG mediated, as determined by protein A-sepharose fractionation. This surprising finding confounds possibilities of using antibodies against whole mosquito midguts as a step in the development of antimosquito vaccines.",
author = "Noden, {B. H.} and Vaughan, {J. A.} and Ibrahim, {M. S.} and Beier, {John C}",
year = "1995",
month = "3",
day = "1",
language = "English",
volume = "11",
pages = "45--49",
journal = "Journal of the American Mosquito Control Association",
issn = "8756-971X",
publisher = "American Mosquito Control Association",
number = "1",

}

TY - JOUR

T1 - An immunological factor that affects Anopheles gambiae survival.

AU - Noden, B. H.

AU - Vaughan, J. A.

AU - Ibrahim, M. S.

AU - Beier, John C

PY - 1995/3/1

Y1 - 1995/3/1

N2 - High titers of antibodies against Anopheles gambiae midguts were produced in New Zealand rabbits to identify midgut targets for an antimosquito vaccine. The serum from one of 8 rabbits (designated R2B6) killed 71.6% (Abbott's adjusted % mortality) of An. gambiae within 7 days. Mosquitoes ingesting R2B6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. Anopheles stephensi and Anopheles arabiensis were also killed when ingesting R2B6 serum but Anopheles freeborni, Anopheles albimanus, and Aedes aegypti were not affected. The mosquitocidal factor was a relatively large molecule (> 100,000 MW) maintained at threshold levels in the sera and killing was complement independent. Mortality, however, was not IgG mediated, as determined by protein A-sepharose fractionation. This surprising finding confounds possibilities of using antibodies against whole mosquito midguts as a step in the development of antimosquito vaccines.

AB - High titers of antibodies against Anopheles gambiae midguts were produced in New Zealand rabbits to identify midgut targets for an antimosquito vaccine. The serum from one of 8 rabbits (designated R2B6) killed 71.6% (Abbott's adjusted % mortality) of An. gambiae within 7 days. Mosquitoes ingesting R2B6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. Anopheles stephensi and Anopheles arabiensis were also killed when ingesting R2B6 serum but Anopheles freeborni, Anopheles albimanus, and Aedes aegypti were not affected. The mosquitocidal factor was a relatively large molecule (> 100,000 MW) maintained at threshold levels in the sera and killing was complement independent. Mortality, however, was not IgG mediated, as determined by protein A-sepharose fractionation. This surprising finding confounds possibilities of using antibodies against whole mosquito midguts as a step in the development of antimosquito vaccines.

UR - http://www.scopus.com/inward/record.url?scp=0029261536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029261536&partnerID=8YFLogxK

M3 - Article

C2 - 7616189

AN - SCOPUS:0029261536

VL - 11

SP - 45

EP - 49

JO - Journal of the American Mosquito Control Association

JF - Journal of the American Mosquito Control Association

SN - 8756-971X

IS - 1

ER -