An experimental model simulating local recurrence and pelvic lymph node metastasis following orthotopic induction of prostate cancer

Johannes Vieweg, Warren D.W. Heston, William R. Fair, Eli Gilboa

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In order to develop an animal model that more closely simulates the organ environment and metastatic routes of human prostatic cancer, R3327-MatLyLu tumors were induced by orthotopic implantation in the ventral prostatic lobe of Copenhagen rats. This procedure reproducibly resulted in metastatic spread of the intraprostatic tumor to the pelvic and retroperitoneal lymph nodes, and invariably to the lungs. Further, a tumor recurrence model was established using an approach that combined orthotopic tumor implantation and subsequent surgical resection of the primary tumor. When prostatectomy was carried out 4 days or more after induction, tumors recurred locally in all animals. The surgical procedures described may provide an animal model to test the in vivo response to experimental adjuvant treatment protocols for advanced prostate cancer. Immunological studies are now in progress using cytokine gene-modified prostatic tumor cells as cellular antitumor vaccines in orthotopically established R3327-MatLyLu tumors.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalThe Prostate
Volume24
Issue number6
DOIs
StatePublished - Jun 1994
Externally publishedYes

Keywords

  • orthotopic implantation
  • prostatic carcinoma
  • tumor recurrence model

ASJC Scopus subject areas

  • Urology

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