An experimental model simulating local recurrence and pelvic lymph node metastasis following orthotopic induction of prostate cancer

J. Vieweg, W. D W Heston, E. Gilboa, Eli Gilboa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In order to develop an animal model that more closely simulates the organ environment and metastatic routes of human prostatic cancer, R3327-MatLyLu tumors were induced by orthotopic implantation in the ventral prostatic lobe of Copenhagen rats. This procedure reproducibly resulted in metastatic spread of the intraprostatic tumor to the pelvic and retroperitoneal lymph nodes, and invariably to the lungs. Further, a tumor recurrence model was established using an approach that combined orthotopic tumor implantation and subsequent surgical resection of the primary tumor. When prostatectomy was carried out 4 days or more after induction, tumors recurred locally in all animals. The surgical procedures described may provide an animal model to test the in vivo response to experimental adjuvant treatment protocols for advanced prostate cancer. Immunological studies are now in progress using cytokine gene-modified prostatic tumor cells as cellular antitumor vaccines in orthotopically established R3327-MatLyLu tumors.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalProstate
Volume24
Issue number6
DOIs
StatePublished - Jun 27 1994
Externally publishedYes

Fingerprint

Prostatic Neoplasms
Theoretical Models
Lymph Nodes
Neoplasm Metastasis
Recurrence
Neoplasms
Animal Models
Clinical Protocols
Prostatectomy
Vaccines
Cytokines
Lung
Genes

Keywords

  • orthotopic implantation
  • prostatic carcinoma
  • tumor recurrence model

ASJC Scopus subject areas

  • Urology

Cite this

An experimental model simulating local recurrence and pelvic lymph node metastasis following orthotopic induction of prostate cancer. / Vieweg, J.; Heston, W. D W; Gilboa, E.; Gilboa, Eli.

In: Prostate, Vol. 24, No. 6, 27.06.1994, p. 291-298.

Research output: Contribution to journalArticle

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