An experimental comparative study of dexamethasone, melatonin and tacrolimus in noise-induced hearing loss

Esperanza Bas Infante, Francisco Martinez-Soriano, José M. Láinez, Jaime Marco

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Conclusion: The calcineurin inhibitor tacrolimus (TCR) and the pineal gland hormone and antioxidant melatonin (MLT) have been shown to possess otoprotective properties against noise-induced hearing loss (NIHL). In contrast, dexamethasone (DXM) was not effective as an otoprotective agent against NIHL. Further studies are needed to understand the exact molecular mechanisms involved. Objective: Exposure to noise pollution and use of audio devices for long periods of time at high volume is known to cause hearing loss or NIHL. Our goal was to evaluate the effectiveness of various known compounds such as the anti-inflammatory DXM, the antioxidant MLT and the immunosuppressant TCR against NIHL. Materials and methods: Thirty-two Wistar rats were randomly divided into groups that were then exposed to intense white noise at 120 dB SPL for 4 h. The day before and for a period of 14 days, test groups were administered one of the three compounds. The efficacy of the compounds against NIHL was determined after examining the shifts in the levels of distortion product otoacoustic emissions (DPOAEs) and changes in the threshold of auditory brainstem responses (ABRs). Cytocochleograms and determination of gene expression in whole rat cochlea were carried out at day 21. Results: Treatment with DXM had no otoprotective effect, while animals treated with MLT experienced an improvement in their hearing functionality. This effect, which is probably linked to MLT's ability to reduce c-fos and TNF- α gene expression thereby preventing outer hair cell (OHC) loss, was even more pronounced in week 3. For its part, TCR provided protection against injury to the cochlea from week 1, eventually leading to a full recovery in hearing. The compound reduced both c-fos and TNF-α expression, as well as OHC loss.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalActa Oto-Laryngologica
Volume129
Issue number4
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Noise-Induced Hearing Loss
Tacrolimus
Melatonin
Dexamethasone
Outer Auditory Hair Cells
Cochlea
Alopecia
Hearing
Antioxidants
fos Genes
Gene Expression
Aptitude
Pineal Gland
Brain Stem Auditory Evoked Potentials
Immunosuppressive Agents
Hearing Loss
Noise
Wistar Rats
Anti-Inflammatory Agents
Hormones

Keywords

  • ABR threshold
  • Anti-inflammatory
  • Antioxidant
  • Calcineurin inhibitor
  • DPOAE
  • Otoprotection
  • Rat

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

An experimental comparative study of dexamethasone, melatonin and tacrolimus in noise-induced hearing loss. / Bas Infante, Esperanza; Martinez-Soriano, Francisco; Láinez, José M.; Marco, Jaime.

In: Acta Oto-Laryngologica, Vol. 129, No. 4, 2009, p. 385-389.

Research output: Contribution to journalArticle

Bas Infante, Esperanza ; Martinez-Soriano, Francisco ; Láinez, José M. ; Marco, Jaime. / An experimental comparative study of dexamethasone, melatonin and tacrolimus in noise-induced hearing loss. In: Acta Oto-Laryngologica. 2009 ; Vol. 129, No. 4. pp. 385-389.
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