An example of lyophilized protein based materials not simulating patient sera

B. W. Steele; D. F. Koehler; T. P. Blaszkowski; M. Azar

doi:10.1093/clinchem/21.12.1812

An example of lyophilized protein based materials not simulating patient sera

B. W. Steele, D. F. Koehler, T. P. Blaszkowski, M. Azar

Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Use of reconstituted lyophilized protein based materials in the clinical laboratory is partly based on the assumption that these materials adequately simulate patients' sera. We examined several of these materials and found that certain ones do not have the same absorbancies at 340 and 380 nm as do most sera. The implication of this is examined with respect to glucose determination by the hexokinase method on a dual wave length blank subtraction instrument.

Original language	English (US)
Pages (from-to)	1812-1814
Number of pages	3
Journal	Clinical chemistry
Volume	21
Issue number	12
DOIs	https://doi.org/10.1093/clinchem/21.12.1812
State	Published - 1975

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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abstract = "Use of reconstituted lyophilized protein based materials in the clinical laboratory is partly based on the assumption that these materials adequately simulate patients' sera. We examined several of these materials and found that certain ones do not have the same absorbancies at 340 and 380 nm as do most sera. The implication of this is examined with respect to glucose determination by the hexokinase method on a dual wave length blank subtraction instrument.",

author = "Steele, {B. W.} and Koehler, {D. F.} and Blaszkowski, {T. P.} and M. Azar",

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