An evaluation of purity criteria for bovine rod outer segment membranes

C. Salesse, F. Boucher, Roger Leblanc

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The purity criteria of bovine rod outer segments (ROS) purified by different procedures were evaluated. Bovine ROS were purified by flotation and/or sedimentation in a continuous concentration gradient of sucrose. The purity of the different fractions was then evaluated according to four purity criteria: (i) the A280 ΔA500 ratio, (ii) the moles of phospholipid per mole of rhodopsin, (iii) the fatty acid composition, and (iv) the interfacial properties of ROS membranes. All the purity criteria, except the A280 ΔA500 ratio, were found to be adequate. From our results, the A280 ΔA500 ratio cannot be used alone to characterize ROS purity. Furthermore, the phospholipid-to-rhodopsin ratio appears as the best purity criterion because of its reliability, its higher sensitivity, and its ease of achievement. It is noteworthy that mechanical treatment of the retinas dramatically affects the purification of ROS.

Original languageEnglish
Pages (from-to)258-266
Number of pages9
JournalAnalytical Biochemistry
Volume142
Issue number2
DOIs
StatePublished - Nov 1 1984
Externally publishedYes

Fingerprint

Rod Cell Outer Segment
Rhodopsin
Phospholipids
Membranes
Flotation
Sedimentation
Purification
Sucrose
Fatty Acids
Chemical analysis
Retina

Keywords

  • fatty acids
  • phospholipids
  • purity criteria
  • rhodopsin
  • rod outer segments
  • visual membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

An evaluation of purity criteria for bovine rod outer segment membranes. / Salesse, C.; Boucher, F.; Leblanc, Roger.

In: Analytical Biochemistry, Vol. 142, No. 2, 01.11.1984, p. 258-266.

Research output: Contribution to journalArticle

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