Abstract
The purity criteria of bovine rod outer segments (ROS) purified by different procedures were evaluated. Bovine ROS were purified by flotation and/or sedimentation in a continuous concentration gradient of sucrose. The purity of the different fractions was then evaluated according to four purity criteria: (i) the A280 ΔA500 ratio, (ii) the moles of phospholipid per mole of rhodopsin, (iii) the fatty acid composition, and (iv) the interfacial properties of ROS membranes. All the purity criteria, except the A280 ΔA500 ratio, were found to be adequate. From our results, the A280 ΔA500 ratio cannot be used alone to characterize ROS purity. Furthermore, the phospholipid-to-rhodopsin ratio appears as the best purity criterion because of its reliability, its higher sensitivity, and its ease of achievement. It is noteworthy that mechanical treatment of the retinas dramatically affects the purification of ROS.
| Original language | English |
|---|---|
| Pages (from-to) | 258-266 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 142 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 1 1984 |
| Externally published | Yes |
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Keywords
- fatty acids
- phospholipids
- purity criteria
- rhodopsin
- rod outer segments
- visual membranes
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
An evaluation of purity criteria for bovine rod outer segment membranes. / Salesse, C.; Boucher, F.; Leblanc, Roger.
In: Analytical Biochemistry, Vol. 142, No. 2, 01.11.1984, p. 258-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An evaluation of purity criteria for bovine rod outer segment membranes
AU - Salesse, C.
AU - Boucher, F.
AU - Leblanc, Roger
PY - 1984/11/1
Y1 - 1984/11/1
N2 - The purity criteria of bovine rod outer segments (ROS) purified by different procedures were evaluated. Bovine ROS were purified by flotation and/or sedimentation in a continuous concentration gradient of sucrose. The purity of the different fractions was then evaluated according to four purity criteria: (i) the A280 ΔA500 ratio, (ii) the moles of phospholipid per mole of rhodopsin, (iii) the fatty acid composition, and (iv) the interfacial properties of ROS membranes. All the purity criteria, except the A280 ΔA500 ratio, were found to be adequate. From our results, the A280 ΔA500 ratio cannot be used alone to characterize ROS purity. Furthermore, the phospholipid-to-rhodopsin ratio appears as the best purity criterion because of its reliability, its higher sensitivity, and its ease of achievement. It is noteworthy that mechanical treatment of the retinas dramatically affects the purification of ROS.
AB - The purity criteria of bovine rod outer segments (ROS) purified by different procedures were evaluated. Bovine ROS were purified by flotation and/or sedimentation in a continuous concentration gradient of sucrose. The purity of the different fractions was then evaluated according to four purity criteria: (i) the A280 ΔA500 ratio, (ii) the moles of phospholipid per mole of rhodopsin, (iii) the fatty acid composition, and (iv) the interfacial properties of ROS membranes. All the purity criteria, except the A280 ΔA500 ratio, were found to be adequate. From our results, the A280 ΔA500 ratio cannot be used alone to characterize ROS purity. Furthermore, the phospholipid-to-rhodopsin ratio appears as the best purity criterion because of its reliability, its higher sensitivity, and its ease of achievement. It is noteworthy that mechanical treatment of the retinas dramatically affects the purification of ROS.
KW - fatty acids
KW - phospholipids
KW - purity criteria
KW - rhodopsin
KW - rod outer segments
KW - visual membranes
UR - http://www.scopus.com/inward/record.url?scp=0021746634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021746634&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(84)90462-7
DO - 10.1016/0003-2697(84)90462-7
M3 - Article
C2 - 6528967
AN - SCOPUS:0021746634
VL - 142
SP - 258
EP - 266
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -