An enzymatic approach to lipoprotein quantification

Bernard W Steele, D. F. Koehler, K. Kuba, M. M. Azar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lipoprotein cholesterol levels were determined without ultracentrifugation by using modified enzymatic methods for cholesterol, high-density-lipoprotein (HDL) cholesterol, and triglyceride and the formula, low-density-lipoprotein (LDL) cholesterol = total cholesterol - HDL cholesterol - triglycerides/5. The methods for cholesterol and triglyceride determinations were standardized for accuracy and precision by the Center for Disease Control's Lipid Standardization Laboratory, which monitored this laboratory for 16 months. The lipoprotein cholesterol values obtained correlated well with lipoprotein cholesterol values determined at the Minnesota Lipid Research Clinic Laboratory using ultracentrifugation. LDL cholesterol determined at the Minneapolis Veterans Administration Hospital Laboratories (Y axis) produced a curve with an intercept of 9.38 mg/dl, a slope of .977, standard error of the estimate (Sy.x) of 8.8 mg/dl, and a correlation coefficient (r) of .983 (n = 32). HDL cholesterol was Y = 0.998 X + .89 mg/dl, Sy.x = 1.6 mg/dl (r = .984, n = 53), and very-low-density-lipoprotein (VLDL) cholesterol was Y = 1.010 X - 1.32 mg/dl, Sy.x = 1.3 mg/dl (r = .996, n = 54).

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume73
Issue number1
StatePublished - Jan 1 1980
Externally publishedYes

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HDL Cholesterol
Lipoproteins
Ultracentrifugation
Cholesterol
LDL Cholesterol
Triglycerides
Veterans Hospitals
Lipids
VLDL Cholesterol
Hospital Laboratories
United States Department of Veterans Affairs
Centers for Disease Control and Prevention (U.S.)
Research
lipoprotein cholesterol
lipoprotein triglyceride

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Steele, B. W., Koehler, D. F., Kuba, K., & Azar, M. M. (1980). An enzymatic approach to lipoprotein quantification. American Journal of Clinical Pathology, 73(1), 75-78.

An enzymatic approach to lipoprotein quantification. / Steele, Bernard W; Koehler, D. F.; Kuba, K.; Azar, M. M.

In: American Journal of Clinical Pathology, Vol. 73, No. 1, 01.01.1980, p. 75-78.

Research output: Contribution to journalArticle

Steele, BW, Koehler, DF, Kuba, K & Azar, MM 1980, 'An enzymatic approach to lipoprotein quantification', American Journal of Clinical Pathology, vol. 73, no. 1, pp. 75-78.
Steele BW, Koehler DF, Kuba K, Azar MM. An enzymatic approach to lipoprotein quantification. American Journal of Clinical Pathology. 1980 Jan 1;73(1):75-78.
Steele, Bernard W ; Koehler, D. F. ; Kuba, K. ; Azar, M. M. / An enzymatic approach to lipoprotein quantification. In: American Journal of Clinical Pathology. 1980 ; Vol. 73, No. 1. pp. 75-78.
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