An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca2+ concentration

Sergei V. Zaitsev; Mats Andersson; Alexandre M. Efanov; Ioulia B. Efanova; Claes Göran Östenson; Lisa Juntti-Berggren; Per Olof Berggren; Viktor Mutt; Suad Efendić

doi:10.1016/S0014-5793(98)01383-0

An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca²⁺ concentration

Sergei V. Zaitsev, Mats Andersson, Alexandre M. Efanov, Ioulia B. Efanova, Claes Göran Östenson, Lisa Juntti-Berggren, Per Olof Berggren, Viktor Mutt, Suad Efendić

Surgery

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4 Scopus citations

Abstract

We have recently isolated and cloned a novel endogenous peptide from pig intestine, NK-lysin (NKL). In the present study we show that NKL (1-100 nM) potently and reversibly stimulates insulin secretion in rat pancreatic islets and in the β-cell line HIT T15. This effect of NKL was not accompanied by changes in cytoplasmic free calcium concentration. The stimulatory activity of NKL on insulin release was also observed in permeabilized islets under Ca²⁺-clamped conditions. Preincubation of HIT T15 cells with NKL for 1 h or 24 h did not influence cell viability. Possible mechanisms of insulinotropic activity of NKL are discussed. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	267-270
Number of pages	4
Journal	FEBS letters
Volume	439
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)01383-0
State	Published - Nov 20 1998

Keywords

Cytoplasmic free Ca concentration
Exocytosis
Insulin release
NK-lysin
Pancreatic β-cell

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Zaitsev, S. V., Andersson, M., Efanov, A. M., Efanova, I. B., Östenson, C. G., Juntti-Berggren, L., Berggren, P. O., Mutt, V., & Efendić, S. (1998). An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca²⁺ concentration. FEBS letters, 439(3), 267-270. https://doi.org/10.1016/S0014-5793(98)01383-0

An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca²⁺ concentration. / Zaitsev, Sergei V.; Andersson, Mats; Efanov, Alexandre M.; Efanova, Ioulia B.; Östenson, Claes Göran; Juntti-Berggren, Lisa; Berggren, Per Olof; Mutt, Viktor; Efendić, Suad.

In: FEBS letters, Vol. 439, No. 3, 20.11.1998, p. 267-270.

Research output: Contribution to journal › Article

Zaitsev, Sergei V. ; Andersson, Mats ; Efanov, Alexandre M. ; Efanova, Ioulia B. ; Östenson, Claes Göran ; Juntti-Berggren, Lisa ; Berggren, Per Olof ; Mutt, Viktor ; Efendić, Suad. / An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca²⁺ concentration. In: FEBS letters. 1998 ; Vol. 439, No. 3. pp. 267-270.

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abstract = "We have recently isolated and cloned a novel endogenous peptide from pig intestine, NK-lysin (NKL). In the present study we show that NKL (1-100 nM) potently and reversibly stimulates insulin secretion in rat pancreatic islets and in the β-cell line HIT T15. This effect of NKL was not accompanied by changes in cytoplasmic free calcium concentration. The stimulatory activity of NKL on insulin release was also observed in permeabilized islets under Ca2+-clamped conditions. Preincubation of HIT T15 cells with NKL for 1 h or 24 h did not influence cell viability. Possible mechanisms of insulinotropic activity of NKL are discussed. Copyright (C) 1998 Federation of European Biochemical Societies.",

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AU - Östenson, Claes Göran

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