An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca2+ concentration

Sergei V. Zaitsev, Mats Andersson, Alexandre M. Efanov, Ioulia B. Efanova, Claes Göran Östenson, Lisa Juntti-Berggren, Per Olof Berggren, Viktor Mutt, Suad Efendić

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have recently isolated and cloned a novel endogenous peptide from pig intestine, NK-lysin (NKL). In the present study we show that NKL (1-100 nM) potently and reversibly stimulates insulin secretion in rat pancreatic islets and in the β-cell line HIT T15. This effect of NKL was not accompanied by changes in cytoplasmic free calcium concentration. The stimulatory activity of NKL on insulin release was also observed in permeabilized islets under Ca2+-clamped conditions. Preincubation of HIT T15 cells with NKL for 1 h or 24 h did not influence cell viability. Possible mechanisms of insulinotropic activity of NKL are discussed. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)267-270
Number of pages4
JournalFEBS letters
Volume439
Issue number3
DOIs
StatePublished - Nov 20 1998

Keywords

  • Cytoplasmic free Ca concentration
  • Exocytosis
  • Insulin release
  • NK-lysin
  • Pancreatic β-cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Zaitsev, S. V., Andersson, M., Efanov, A. M., Efanova, I. B., Östenson, C. G., Juntti-Berggren, L., Berggren, P. O., Mutt, V., & Efendić, S. (1998). An endogenous peptide isolated from the gut, NK-lysin, stimulates insulin secretion without changes in cytosolic free Ca2+ concentration. FEBS letters, 439(3), 267-270. https://doi.org/10.1016/S0014-5793(98)01383-0