An efficient method for eccrine gland isolation from human scalp

Francisco Jimenez, Majid Alam, Irene Hernandez, Enrique Poblet, Jonathan A. Hardman, Ralf Paus

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We describe a simple and efficient method to isolate eccrine sweat glands from the human scalp. This method is inspired by the hair graft harvesting method used in hair transplantation. Based on the recently described anatomical relationship between the scalp hair follicle and the eccrine gland, we have found that scalp follicular unit grafts are an excellent eccrine gland isolation source, especially for the coiled component. In order to make the gland visible for stereoscopic microdissection, the follicular units need to be previously stained with a vital dye like methylene blue or neutral red. The simplicity and efficiency of this isolation method should encourage further research into human eccrine sweat gland function which has always been hindered by the difficulty of gland isolation.

Original languageEnglish (US)
Pages (from-to)678-681
Number of pages4
JournalExperimental dermatology
Issue number6
StatePublished - Jun 2018


  • Eccrine glands
  • follicular unit
  • hair follicle
  • hair graft
  • hair transplantation
  • methylene blue
  • neutral red
  • sweat
  • sweat glands

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology


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