An autoradiographic study of the effects of mammalian gonadotropins (follicle-stimulating hormone and luteinizing hormone) and estradiol-17β on [3H]thymidine labeling of surface epithelial cells, prefollicular cells, and oogonia in the ovary of the lizard Anolis carolinensis

Richard R. Tokarz

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Adult, hypophysectomized Anolis carolinensis with seasonally quiescent ovaries were treated with daily, subcutaneous injections of either 0.05 ml of saline, 10 μg of ovine follicle-stimulating hormone (FSH), 10 μg of ovine luteinizing hormone (LH), or 1 μg of estradiol-17β for 5 days. One hour before sacrifice, each animal received an intraperitoneal injection of 3 μCi of [methyl-3H]thymidine. After sacrifice, the ovaries were removed and serially sectioned. Autoradiographs of the region of the ovarian cortex consisting of a surface epithelium, prefollicular cells, oogonia, and primary oocytes (i.e., germinal bed) were then prepared and examined microscopically. Treatment with FSH increased significantly the number of [3H]thymidine-labeled surface epithelial cells, prefollicular cells, and oogonia, as well as the total number of oogonia and the oogonial labeling index, i.e., the ratio of labeled oogonia to total oogonia × 100. Neither LH nor estradiol-17β treatments had a significant effect on these parameters. These results suggest that an FSH-like gonadotropin may mediate cellular proliferation in the germinal bed of this lizard.

Original languageEnglish (US)
Pages (from-to)179-188
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume35
Issue number2
DOIs
StatePublished - Jun 1978

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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