An autoradiographic study of the effects of mammalian gonadotropins (follicle-stimulating hormone and luteinizing hormone) and estradiol-17β on [3H]thymidine labeling of surface epithelial cells, prefollicular cells, and oogonia in the ovary of the lizard Anolis carolinensis

Richard Tokarz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Adult, hypophysectomized Anolis carolinensis with seasonally quiescent ovaries were treated with daily, subcutaneous injections of either 0.05 ml of saline, 10 μg of ovine follicle-stimulating hormone (FSH), 10 μg of ovine luteinizing hormone (LH), or 1 μg of estradiol-17β for 5 days. One hour before sacrifice, each animal received an intraperitoneal injection of 3 μCi of [methyl-3H]thymidine. After sacrifice, the ovaries were removed and serially sectioned. Autoradiographs of the region of the ovarian cortex consisting of a surface epithelium, prefollicular cells, oogonia, and primary oocytes (i.e., germinal bed) were then prepared and examined microscopically. Treatment with FSH increased significantly the number of [3H]thymidine-labeled surface epithelial cells, prefollicular cells, and oogonia, as well as the total number of oogonia and the oogonial labeling index, i.e., the ratio of labeled oogonia to total oogonia × 100. Neither LH nor estradiol-17β treatments had a significant effect on these parameters. These results suggest that an FSH-like gonadotropin may mediate cellular proliferation in the germinal bed of this lizard.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume35
Issue number2
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

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Oogonia
Anolis carolinensis
oogonia
Lizards
follicle-stimulating hormone
Follicle Stimulating Hormone
gonadotropins
luteinizing hormone
Luteinizing Hormone
thymidine
Gonadotropins
Thymidine
lizards
estradiol
Ovary
Estradiol
epithelial cells
Epithelial Cells
cells
Sheep

ASJC Scopus subject areas

  • Endocrinology

Cite this

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title = "An autoradiographic study of the effects of mammalian gonadotropins (follicle-stimulating hormone and luteinizing hormone) and estradiol-17β on [3H]thymidine labeling of surface epithelial cells, prefollicular cells, and oogonia in the ovary of the lizard Anolis carolinensis",
abstract = "Adult, hypophysectomized Anolis carolinensis with seasonally quiescent ovaries were treated with daily, subcutaneous injections of either 0.05 ml of saline, 10 μg of ovine follicle-stimulating hormone (FSH), 10 μg of ovine luteinizing hormone (LH), or 1 μg of estradiol-17β for 5 days. One hour before sacrifice, each animal received an intraperitoneal injection of 3 μCi of [methyl-3H]thymidine. After sacrifice, the ovaries were removed and serially sectioned. Autoradiographs of the region of the ovarian cortex consisting of a surface epithelium, prefollicular cells, oogonia, and primary oocytes (i.e., germinal bed) were then prepared and examined microscopically. Treatment with FSH increased significantly the number of [3H]thymidine-labeled surface epithelial cells, prefollicular cells, and oogonia, as well as the total number of oogonia and the oogonial labeling index, i.e., the ratio of labeled oogonia to total oogonia × 100. Neither LH nor estradiol-17β treatments had a significant effect on these parameters. These results suggest that an FSH-like gonadotropin may mediate cellular proliferation in the germinal bed of this lizard.",
author = "Richard Tokarz",
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