Adult, hypophysectomized Anolis carolinensis with seasonally quiescent ovaries were treated with daily, subcutaneous injections of either 0.05 ml saline, 1 μg estradiol-17β, or 10 μg ovine follicle-stimulating hormone (FSH) for 48 or 96 hr. One hour before sacrifice, each animal received an intraperitoneal injection of 3 μCi 3H-thymidine. Autoradiographs of the serially sectioned ovaries were prepared and the percentage labeling index (%LI), which is the ratio of labeled cells to all cells in the sample × 100, was determined. After FSH treatment, the %LI was significantly higher in the ovarian medullary stroma, the basal granulosa cells in larger previtellogenic follicles (0.51-1.00 mm), and the theca of all follicular size classes present (0.01-1.00 mm). Estradiol had no significant effect on these parameters. These results are discussed in relation to the effects of FSH and estradiol on follicular maturation in reptiles.
ASJC Scopus subject areas
- Animal Science and Zoology