An autoradiographic study of the effects of FSH and estradiol-17β on early ovarian follicular maturation in adult Anolis carolinensis

Richard Tokarz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Adult, hypophysectomized Anolis carolinensis with seasonally quiescent ovaries were treated with daily, subcutaneous injections of either 0.05 ml saline, 1 μg estradiol-17β, or 10 μg ovine follicle-stimulating hormone (FSH) for 48 or 96 hr. One hour before sacrifice, each animal received an intraperitoneal injection of 3 μCi 3H-thymidine. Autoradiographs of the serially sectioned ovaries were prepared and the percentage labeling index (%LI), which is the ratio of labeled cells to all cells in the sample × 100, was determined. After FSH treatment, the %LI was significantly higher in the ovarian medullary stroma, the basal granulosa cells in larger previtellogenic follicles (0.51-1.00 mm), and the theca of all follicular size classes present (0.01-1.00 mm). Estradiol had no significant effect on these parameters. These results are discussed in relation to the effects of FSH and estradiol on follicular maturation in reptiles.

Original languageEnglish
Pages (from-to)17-28
Number of pages12
JournalGeneral and Comparative Endocrinology
Volume31
Issue number1
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

Fingerprint

Anolis carolinensis
follicle-stimulating hormone
Follicle Stimulating Hormone
estradiol
Estradiol
Ovary
Reptiles
Granulosa Cells
granulosa cells
autoradiography
subcutaneous injection
Subcutaneous Injections
intraperitoneal injection
thymidine
Intraperitoneal Injections
Thymidine
reptiles
Sheep
cells
sheep

ASJC Scopus subject areas

  • Endocrinology

Cite this

An autoradiographic study of the effects of FSH and estradiol-17β on early ovarian follicular maturation in adult Anolis carolinensis. / Tokarz, Richard.

In: General and Comparative Endocrinology, Vol. 31, No. 1, 01.01.1977, p. 17-28.

Research output: Contribution to journalArticle

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