An approach to immunomicroscopy of renal disease with immunoperoxidase and periodic acid-Schiff counterstain (IMPAS stain)

R. R. Tubbs, G. Gephardt, R. Valenzuela, S. Deodhar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A series of 127 renal specimens reflecting a wide spectrum of renal pathology was evaluated with a direct immunoperoxidase technic in which frozen tissue was first reacted with anti-Ig(G,A,M) and anti-C3-peroxidase conjugates and subsequently counterstained with periodic acid-Schiff-hematoxylin (IMPAS). This technic permitted simultaneous evaluation of light-microscopic changes and yielded a permanent immunomicroscopic record. Frequently, a distinction could be made between epimembranous, intramembranous and subendothelial deposits, which was not possible using immunofluorescence. The findings were correlated with histologic, immunofluorescent and electronmicroscopic results. General patterns of immune complex and autoantibody deposition were similar in 124 cases. In one case, there was poor correlation between results obtained by immunoperoxidase and immunofluorescence. In two other cases, deposits detected by electron microscopy were not identified by either immunoperoxidase or immunofluorescence. The IMPAS stain, combining immunomicroscopy with conventional light microscopy, may be a useful approach to the laboratory diagnosis of renal disease.

Original languageEnglish
Pages (from-to)240-244
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume73
Issue number2
StatePublished - Jan 1 1980
Externally publishedYes

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Periodic Acid
Fluorescent Antibody Technique
Kidney
Light
Clinical Laboratory Techniques
Hematoxylin
Antigen-Antibody Complex
Immunoenzyme Techniques
Autoantibodies
Peroxidase
Microscopy
Electron Microscopy
Pathology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

An approach to immunomicroscopy of renal disease with immunoperoxidase and periodic acid-Schiff counterstain (IMPAS stain). / Tubbs, R. R.; Gephardt, G.; Valenzuela, R.; Deodhar, S.

In: American Journal of Clinical Pathology, Vol. 73, No. 2, 01.01.1980, p. 240-244.

Research output: Contribution to journalArticle

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