An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation

J. Madison Clark, George Brinson, Michael K. Newman, Brian S. Jewett, Balfour R. Sartor, Jiri Prazma, Harold C. Pillsbury

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: Chronic otitis media with effusion (COME) is the most prevalent inflammatory disease in children and is associated with numerous adverse long-term sequelae. Many factors have been associated with an increased risk of developing COME, one of which may be a genetic predisposition to the disease. To study the role that genetics play in the pathogenesis of COME, we used an animal model to compare the middle ear inflammatory responses in two different strains of rats (Lewis and Fisher). Methods: In earlier studies, we demonstrated that exposure of the middle ear to endotoxin caused early extensive exudation and, later, goblet cell hyperplasia and mucin hypersecretion. In the present study, the animals were divided into six groups. In each group the animals were given transtympanic injection with gram-positive bacterial cell wall product (peptidoglycan-polysaccharide [PG-PS]). The middle ear bullae were studied at 1 week and 3 weeks after infection, and after systemic reinfection. Comparisons were made of the quantity of mucin exudate by enzyme-linked immunosorbent assay and by histological evaluation of the middle ear epithelial thickness. Results: Our data demonstrate a statistically significant difference in middle ear inflammation and effusion formation between the two genetically different strains of rats. Conclusions: These data support the hypothesis that the middle ear response to PG-PS may be genetically determined and therefore suggest that genetic predisposition may play a role in the pathogenesis of COME.

Original languageEnglish
Pages (from-to)1511-1515
Number of pages5
JournalLaryngoscope
Volume110
Issue number9
StatePublished - Sep 25 2000
Externally publishedYes

Fingerprint

Otitis Media with Effusion
Otitis Media
Middle Ear
Genetic Predisposition to Disease
Animal Models
Peptidoglycan
Mucins
Polysaccharides
Goblet Cells
Exudates and Transudates
Blister
Endotoxins
Cell Wall
Hyperplasia
Enzyme-Linked Immunosorbent Assay
Injections
Infection

Keywords

  • Chronic otitis media
  • Genetic predisposition
  • Middle ear inflammation
  • PG-PS.

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Clark, J. M., Brinson, G., Newman, M. K., Jewett, B. S., Sartor, B. R., Prazma, J., & Pillsbury, H. C. (2000). An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation. Laryngoscope, 110(9), 1511-1515.

An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation. / Clark, J. Madison; Brinson, George; Newman, Michael K.; Jewett, Brian S.; Sartor, Balfour R.; Prazma, Jiri; Pillsbury, Harold C.

In: Laryngoscope, Vol. 110, No. 9, 25.09.2000, p. 1511-1515.

Research output: Contribution to journalArticle

Clark, JM, Brinson, G, Newman, MK, Jewett, BS, Sartor, BR, Prazma, J & Pillsbury, HC 2000, 'An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation', Laryngoscope, vol. 110, no. 9, pp. 1511-1515.
Clark JM, Brinson G, Newman MK, Jewett BS, Sartor BR, Prazma J et al. An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation. Laryngoscope. 2000 Sep 25;110(9):1511-1515.
Clark, J. Madison ; Brinson, George ; Newman, Michael K. ; Jewett, Brian S. ; Sartor, Balfour R. ; Prazma, Jiri ; Pillsbury, Harold C. / An animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation. In: Laryngoscope. 2000 ; Vol. 110, No. 9. pp. 1511-1515.
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