Abstract
The human oncogene β-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/β-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of β-catenin remains unclear. In many animal embryos, activation of β-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of β-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of β-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of β-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis β-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation.
| Original language | English |
|---|---|
| Pages (from-to) | 446-450 |
| Number of pages | 5 |
| Journal | Nature |
| Volume | 426 |
| Issue number | 6965 |
| DOIs | |
| State | Published - Nov 27 2003 |
| Externally published | Yes |
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Cite this
An ancient role for nuclear β-catenin in the evolution of axial polarity and germ layer segregation. / Wikramanayake, Athula; Hong, Melanie; Lee, Patricia N.; Pang, Kevin; Byrum, Christine A.; Bince, Joanna M.; Xu, Ronghui; Martindale, Mark Q.
In: Nature, Vol. 426, No. 6965, 27.11.2003, p. 446-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An ancient role for nuclear β-catenin in the evolution of axial polarity and germ layer segregation
AU - Wikramanayake, Athula
AU - Hong, Melanie
AU - Lee, Patricia N.
AU - Pang, Kevin
AU - Byrum, Christine A.
AU - Bince, Joanna M.
AU - Xu, Ronghui
AU - Martindale, Mark Q.
PY - 2003/11/27
Y1 - 2003/11/27
N2 - The human oncogene β-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/β-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of β-catenin remains unclear. In many animal embryos, activation of β-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of β-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of β-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of β-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis β-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation.
AB - The human oncogene β-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/β-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of β-catenin remains unclear. In many animal embryos, activation of β-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of β-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of β-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of β-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis β-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0344120305&partnerID=8YFLogxK
U2 - 10.1038/nature02113
DO - 10.1038/nature02113
M3 - Article
C2 - 14647383
AN - SCOPUS:0344120305
VL - 426
SP - 446
EP - 450
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6965
ER -