Amphetamine potentiates the effects of β-phenylethylamine through activation of an amine-gated chloride channel

Bryan D. Safratowich, Murad Hossain, Laura Bianchi, Lucia Carvelli

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

β-Phenylethylamine (βPEA) is a trace amine present in the CNS of all animals tested to date. However, its function is still not fully understood. βPEA has been suggested to function as a neurotransmitter and/or to mimic the effect of amphetamine (Amph). In support of the latter is the observation that βPEA and Amph produce similar but not identical behaviors. Here, we show that βPEA, like Amph, activates the dopamine transporter and the amine-gated chloride channel LGC-55 to generate behaviors in Caenorhabditis elegans. However, although Amph-induced behaviors occurred gradually during 10 min of treatment, βPEA induced maximal effects within 1 min. In vitro data demonstrate that βPEA activates the LGC-55 more efficiently than Amph (K m = 9 and 152 μM, respectively) and generates saturating currents that are 10 times larger than those produced by Amph. These results suggest that activation of LGC-55 mostly accounts for the behavioral effects reached after 1 min of treatment with βPEA. Importantly, our in vitro and in vivo data show that Amph increases the effects induced by βPEA on the LGC-55, indicating that Amph potentiates the effects generated by the biogenic amine βPEA. Together, our data not only identify a new target for βPEA, but also offer a novel mechanism of action of Amph. In addition, our results highlight C. elegans as a powerful genetic model for studying the effects of biogenic and synthetic amines both at the molecular and behavioral levels.

Original languageEnglish
Pages (from-to)4686-4691
Number of pages6
JournalJournal of Neuroscience
Volume34
Issue number13
DOIs
StatePublished - Jan 1 2014

Fingerprint

Phenethylamines
Chloride Channels
Amphetamine
Amines
Biogenic Amines
Caenorhabditis elegans
Dopamine Plasma Membrane Transport Proteins
Genetic Models
Neurotransmitter Agents

Keywords

  • Amphetamine
  • Caenorhabditis elegans
  • Dopamine transporter
  • Ligand-gated ion channels
  • β-phenylethylamine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Amphetamine potentiates the effects of β-phenylethylamine through activation of an amine-gated chloride channel. / Safratowich, Bryan D.; Hossain, Murad; Bianchi, Laura; Carvelli, Lucia.

In: Journal of Neuroscience, Vol. 34, No. 13, 01.01.2014, p. 4686-4691.

Research output: Contribution to journalArticle

Safratowich, Bryan D. ; Hossain, Murad ; Bianchi, Laura ; Carvelli, Lucia. / Amphetamine potentiates the effects of β-phenylethylamine through activation of an amine-gated chloride channel. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 13. pp. 4686-4691.
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