Amperometric sensing at high temperature with a "wired" thermostable glucose-6-phosphate dehydrogenase from Aquifex aeolicus

Ramesh Iyer, Valeri Pavlov, Ioanis Katakis, Leonidas G. Bachas

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

An amperometric enzyme sensor capable of operating at high temperatures was developed by utilizing a "wired" thermostable glucose-6-phosphate dehydrogenase (tG6PDH) from the hyperthermophilic bacterium Aquifex aeolicus. The response of the system was monitored through detection of the product of the enzymatic reaction, NADH, which was electrocatalytically reoxidized to NAD by a thermostable redox mediator, osmium (1,10-phenanthroline-5,6-dione)2-poly(4-vinylpyridine), at Eapp = +150 mV vs Ag/AgCl/KClsat. The enzyme was "wired" onto the surface of graphite electrodes by using an epoxy-based poly(ethylene glycol) diglycidyl ether cross-linker. The stability of the sensor at higher temperatures clearly surpassed the conventional system utilizing a mesophilic G6PDH (mG6PDH) from Leuconostoc mesenteroides. The mG6PDH-based system lost 26% of its response after 20 min at 50 °C. The response of the tG6PDH-based system remained unchanged under the same conditions. The tG6PDH-based system demonstrated excellent stability up to a temperature of 83 °C.

Original languageEnglish (US)
Pages (from-to)3898-3901
Number of pages4
JournalAnalytical Chemistry
Volume75
Issue number15
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Amperometric sensing at high temperature with a "wired" thermostable glucose-6-phosphate dehydrogenase from Aquifex aeolicus'. Together they form a unique fingerprint.

  • Cite this