Amniotic fluid-derived extracellular vesicles: characterization and therapeutic efficacy in an experimental model of bronchopulmonary dysplasia

Michael A. Bellio, Karen C. Young, Julian Milberg, Ivan Santos, Zanub Abdullah, Danique Stewart, Alissa Arango, Pingping Chen, Jian Huang, Kevin Williams, Kaitlyn Kelly, Shanique Sterling, Aisha Khan, Xiumin Xu, George C. Shapiro, Maria Ines Mitrani

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background aims: Extracellular vesicles (EVs) are being tested for their use as novel therapeutics. However, the optimal source of EVs is currently under investigation. Amniotic fluid (AF) is a natural source of EVs that can be easily obtained for use in regenerative medicine, yet AF-EV characterization has not been fully explored. Methods: Here the authors demonstrate AF as a rich source of EVs and identify the microRNA and proteomic cargo. Bioinformatics analysis of this cargo revealed multiple pathway targets, including immunomodulatory, anti-inflammatory and free radical scavenging networks. The authors further demonstrated the therapeutic potential of this EV product as a novel preventative agent for bronchopulmonary dysplasia (BPD). Results: Intra-tracheal administration of AF-EVs preserved alveolar development, attenuated vascular remodeling and pulmonary hypertension, decreased lung pro-inflammatory cytokine expression and reduced macrophage infiltration in an experimental BPD model. Conclusions: The authors’ results suggest that AF is a viable biological fluid for EV harvest and that AF-EVs have strong therapeutic potential for pulmonary diseases, such as BPD, warranting further development to transition this novel EV product into the clinic.

Original languageEnglish (US)
Pages (from-to)1097-1107
Number of pages11
JournalCytotherapy
Volume23
Issue number12
DOIs
StatePublished - Dec 2021

Keywords

  • amniotic fluid
  • bronchopulmonary dysplasia
  • extracellular vesicles
  • hyperoxia
  • lung injury
  • pulmonary

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research

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