Ammonia stimulates the release of taurine from cultured astrocytes

Jan Albrecht; Alex S. Bender; Michael D. Norenberg

doi:10.1016/0006-8993(94)91301-3

Ammonia stimulates the release of taurine from cultured astrocytes

Jan Albrecht, Alex S. Bender, Michael D. Norenberg

Pathology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The effect of ammonia on the release of the neuroactive amino acids taurine (TAU), γ-aminobutyric acid (GABA) and d-aspartate (d-ASP), an analog of l-glutamate (l-GLU), from cultured rat cortical astrocytes was studied. NH₄Cl (1 and 5 mM) induced the release of TAU. TAU release was reduced when Na⁺ was removed, and was almost completely abolished when Cl^- was omitted. In contrast, TAU basal release was enhanced upon removal of Na⁺ or Cl^-. Ammonia inhibited the release of GABA and d-ASP. Ammonia-induced release of astroglial TAU may modify the neuronal excitability accompanying hyperammonemic conditions.

Original language	English (US)
Pages (from-to)	288-292
Number of pages	5
Journal	Brain research
Volume	660
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(94)91301-3
State	Published - Oct 17 1994

Keywords

Amino acid release
Ammonium chloride
Astrocytes
GABA
Glutamate
Hepatic encephalopathy
Taurine

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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title = "Ammonia stimulates the release of taurine from cultured astrocytes",

abstract = "The effect of ammonia on the release of the neuroactive amino acids taurine (TAU), γ-aminobutyric acid (GABA) and d-aspartate (d-ASP), an analog of l-glutamate (l-GLU), from cultured rat cortical astrocytes was studied. NH4Cl (1 and 5 mM) induced the release of TAU. TAU release was reduced when Na+ was removed, and was almost completely abolished when Cl- was omitted. In contrast, TAU basal release was enhanced upon removal of Na+ or Cl-. Ammonia inhibited the release of GABA and d-ASP. Ammonia-induced release of astroglial TAU may modify the neuronal excitability accompanying hyperammonemic conditions.",

keywords = "Amino acid release, Ammonium chloride, Astrocytes, GABA, Glutamate, Hepatic encephalopathy, Taurine",

author = "Jan Albrecht and Bender, {Alex S.} and Norenberg, {Michael D.}",

note = "Funding Information: This work was supported by the Veterans Administration, an NIH Biomedical Research Support Grant from the University of Miami, USPHS grants NS-30291 and DK-38153, and GRECC.",

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AU - Bender, Alex S.

AU - Norenberg, Michael D.

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KW - Hepatic encephalopathy

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