TY - JOUR
T1 - Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes
AU - Norenberg, Michael D.
AU - Neary, Joseph T.
AU - Norenberg, Luz Oliva B.
AU - McCarthy, Michelin
PY - 1990/7
Y1 - 1990/7
N2 - Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.
AB - Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.
KW - Ammonia
KW - Astrocytes
KW - Encephalopathy, hepatic
KW - Glial fibrillary acidic protein
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U2 - 10.1097/00005072-199007000-00004
DO - 10.1097/00005072-199007000-00004
M3 - Article
C2 - 2163437
AN - SCOPUS:0025330683
VL - 49
SP - 399
EP - 405
JO - Journal of Neuropathology and Experimental Neurology
JF - Journal of Neuropathology and Experimental Neurology
SN - 0022-3069
IS - 4
ER -