Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes

Michael D. Norenberg; Joseph T. Neary; Luz Oliva B. Norenberg; Michelin McCarthy

doi:10.1097/00005072-199007000-00004

Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes

Michael D. Norenberg, Joseph T. Neary, Luz Oliva B. Norenberg, Michelin McCarthy

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63 Scopus citations

Abstract

Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.

Original language	English (US)
Pages (from-to)	399-405
Number of pages	7
Journal	Journal of Neuropathology and Experimental Neurology
Volume	49
Issue number	4
DOIs	https://doi.org/10.1097/00005072-199007000-00004
State	Published - Jul 1990

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Keywords

Ammonia
Astrocytes
Encephalopathy, hepatic
Glial fibrillary acidic protein

ASJC Scopus subject areas

Pathology and Forensic Medicine
Neuroscience(all)

Cite this

Norenberg, M. D., Neary, J. T., Norenberg, L. O. B., & McCarthy, M. (1990). Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes. Journal of Neuropathology and Experimental Neurology, 49(4), 399-405. https://doi.org/10.1097/00005072-199007000-00004

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10.1097/00005072-199007000-00004