Abstract
Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 399-405 |
| Number of pages | 7 |
| Journal | Journal of Neuropathology and Experimental Neurology |
| Volume | 49 |
| Issue number | 4 |
| State | Published - Dec 1 1990 |
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Keywords
- Ammonia
- Astrocytes
- Encephalopathy, hepatic
- Glial fibrillary acidic protein
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Neuroscience(all)
Cite this
Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes. / Norenberg, Michael D; Neary, Joseph T.; Norenberg, Luz Oliva B; McCarthy, Micheline.
In: Journal of Neuropathology and Experimental Neurology, Vol. 49, No. 4, 01.12.1990, p. 399-405.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes
AU - Norenberg, Michael D
AU - Neary, Joseph T.
AU - Norenberg, Luz Oliva B
AU - McCarthy, Micheline
PY - 1990/12/1
Y1 - 1990/12/1
N2 - Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.
AB - Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.
KW - Ammonia
KW - Astrocytes
KW - Encephalopathy, hepatic
KW - Glial fibrillary acidic protein
UR - http://www.scopus.com/inward/record.url?scp=0025330683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025330683&partnerID=8YFLogxK
M3 - Article
VL - 49
SP - 399
EP - 405
JO - American Journal of Psychotherapy
JF - American Journal of Psychotherapy
SN - 0002-9564
IS - 4
ER -