Ammonia induced decrease in glial fibrillary acidic protein in cultured astrocytes

Michael D. Norenberg, Joseph T. Neary, Luz Oliva B. Norenberg, Michelin McCarthy

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Abstract

Previous studies of human hepatic encephalopathy (HE) have shown decreased levels of glial fibrillary acidic protein (GFAP) in Alzheimer type II astrocytes. In view of the important role of ammonia in the pathogenesis of HE, we carried out immunocytochemical and enzyme-linked immunosorbent assay (ELISA) studies on the effect of ammonium chloride (10 mM) on GFAP content in primary astrocyte cultures. There was a 39% loss of GFAP after a four day treatment. There was no fall in total cell protein. Potential mechanisms for this apparent selective loss of GFAP are discussed.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalJournal of Neuropathology and Experimental Neurology
Volume49
Issue number4
DOIs
StatePublished - Jul 1990

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Keywords

  • Ammonia
  • Astrocytes
  • Encephalopathy, hepatic
  • Glial fibrillary acidic protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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