Ammonia-induced astrocyte swelling in primary culture

Michael D Norenberg, L. Baker, L. O B Norenberg, J. Blicharska, J. H. Bruce-Gregorios, J. T. Neary

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

The effect of ammonia on water space of astrocytes in culture was determined as a means of studying the neurotoxicity of ammonia in fulminant hepatic failure (FHF). Treatment of primary astrocyte cultures obtained from neonatal rat cortices with 10 mM NH4Cl for 4 days resulted in a 29% increase in astrocytic water space, as measured by an isotopic method utilizing 3-O-methyl-[3H]-glucose. this effect was time- and dose-dependent. The ammonia-induced swelling was reversible as the water space in cultures treated with 10 mH NH4Cl for 3 days, and then returned to normal culture media for 1 day, was similar to control cultures. These findings suggest that elevated levels of ammonia lead to astrocyte swelling and may contribute to the brain edema in FHF.

Original languageEnglish
Pages (from-to)833-836
Number of pages4
JournalNeurochemical Research
Volume16
Issue number7
DOIs
StatePublished - Jul 1 1991

Fingerprint

Ammonia
Astrocytes
Swelling
Acute Liver Failure
Water
Brain Edema
Culture Media
Rats
Brain
Glucose

Keywords

  • Ammonia
  • brain edema
  • hepatic encephalopathy
  • hepatic failure

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Norenberg, M. D., Baker, L., Norenberg, L. O. B., Blicharska, J., Bruce-Gregorios, J. H., & Neary, J. T. (1991). Ammonia-induced astrocyte swelling in primary culture. Neurochemical Research, 16(7), 833-836. https://doi.org/10.1007/BF00965694

Ammonia-induced astrocyte swelling in primary culture. / Norenberg, Michael D; Baker, L.; Norenberg, L. O B; Blicharska, J.; Bruce-Gregorios, J. H.; Neary, J. T.

In: Neurochemical Research, Vol. 16, No. 7, 01.07.1991, p. 833-836.

Research output: Contribution to journalArticle

Norenberg, MD, Baker, L, Norenberg, LOB, Blicharska, J, Bruce-Gregorios, JH & Neary, JT 1991, 'Ammonia-induced astrocyte swelling in primary culture', Neurochemical Research, vol. 16, no. 7, pp. 833-836. https://doi.org/10.1007/BF00965694
Norenberg MD, Baker L, Norenberg LOB, Blicharska J, Bruce-Gregorios JH, Neary JT. Ammonia-induced astrocyte swelling in primary culture. Neurochemical Research. 1991 Jul 1;16(7):833-836. https://doi.org/10.1007/BF00965694
Norenberg, Michael D ; Baker, L. ; Norenberg, L. O B ; Blicharska, J. ; Bruce-Gregorios, J. H. ; Neary, J. T. / Ammonia-induced astrocyte swelling in primary culture. In: Neurochemical Research. 1991 ; Vol. 16, No. 7. pp. 833-836.
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