Abstract
The effect of ammonia on water space of astrocytes in culture was determined as a means of studying the neurotoxicity of ammonia in fulminant hepatic failure (FHF). Treatment of primary astrocyte cultures obtained from neonatal rat cortices with 10 mM NH4Cl for 4 days resulted in a 29% increase in astrocytic water space, as measured by an isotopic method utilizing 3-O-methyl-[3H]-glucose. this effect was time- and dose-dependent. The ammonia-induced swelling was reversible as the water space in cultures treated with 10 mH NH4Cl for 3 days, and then returned to normal culture media for 1 day, was similar to control cultures. These findings suggest that elevated levels of ammonia lead to astrocyte swelling and may contribute to the brain edema in FHF.
| Original language | English |
|---|---|
| Pages (from-to) | 833-836 |
| Number of pages | 4 |
| Journal | Neurochemical Research |
| Volume | 16 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 1991 |
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Keywords
- Ammonia
- brain edema
- hepatic encephalopathy
- hepatic failure
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry
Cite this
Ammonia-induced astrocyte swelling in primary culture. / Norenberg, Michael D; Baker, L.; Norenberg, L. O B; Blicharska, J.; Bruce-Gregorios, J. H.; Neary, J. T.
In: Neurochemical Research, Vol. 16, No. 7, 01.07.1991, p. 833-836.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ammonia-induced astrocyte swelling in primary culture
AU - Norenberg, Michael D
AU - Baker, L.
AU - Norenberg, L. O B
AU - Blicharska, J.
AU - Bruce-Gregorios, J. H.
AU - Neary, J. T.
PY - 1991/7/1
Y1 - 1991/7/1
N2 - The effect of ammonia on water space of astrocytes in culture was determined as a means of studying the neurotoxicity of ammonia in fulminant hepatic failure (FHF). Treatment of primary astrocyte cultures obtained from neonatal rat cortices with 10 mM NH4Cl for 4 days resulted in a 29% increase in astrocytic water space, as measured by an isotopic method utilizing 3-O-methyl-[3H]-glucose. this effect was time- and dose-dependent. The ammonia-induced swelling was reversible as the water space in cultures treated with 10 mH NH4Cl for 3 days, and then returned to normal culture media for 1 day, was similar to control cultures. These findings suggest that elevated levels of ammonia lead to astrocyte swelling and may contribute to the brain edema in FHF.
AB - The effect of ammonia on water space of astrocytes in culture was determined as a means of studying the neurotoxicity of ammonia in fulminant hepatic failure (FHF). Treatment of primary astrocyte cultures obtained from neonatal rat cortices with 10 mM NH4Cl for 4 days resulted in a 29% increase in astrocytic water space, as measured by an isotopic method utilizing 3-O-methyl-[3H]-glucose. this effect was time- and dose-dependent. The ammonia-induced swelling was reversible as the water space in cultures treated with 10 mH NH4Cl for 3 days, and then returned to normal culture media for 1 day, was similar to control cultures. These findings suggest that elevated levels of ammonia lead to astrocyte swelling and may contribute to the brain edema in FHF.
KW - Ammonia
KW - brain edema
KW - hepatic encephalopathy
KW - hepatic failure
UR - http://www.scopus.com/inward/record.url?scp=0025882742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025882742&partnerID=8YFLogxK
U2 - 10.1007/BF00965694
DO - 10.1007/BF00965694
M3 - Article
C2 - 1944774
AN - SCOPUS:0025882742
VL - 16
SP - 833
EP - 836
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 7
ER -