Ammonia and urea excretion in the tidepool sculpin (Oligocottus maculosus): sites of excretion, effects of reduced salinity and mechanisms of urea transport

P. A. Wright, P. Part, C. M. Wood

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Tidepool sculpins live in a variable environment where water temperature, salinity, gas tensions, and pH can change considerably with the daily tide cycle. Tidepool sculpins are primarily ammoniotelic, with 8-17% of nitrogen wastes excreted as urea. The majority of net ammonia (Jnetamm; 85%) and urea (Jneturea; 74%) excretion occurred across the gill, with the remainder excreted across the skin, the kidney, and/or gut. Acute (2h) exposure to 50% seawater significantly increased Jneturea (2.8-fold), but reduced Jnetamm (3.5-fold). In fish exposed to 50% seawater for 1 week, Jneturea returned to control values, but Jnetamm remained slightly depressed. Unidirectional urea influx (Jinurea) and efflux (Jouturea) were measured using14C-urea to determine if urea was excreted across the gills by simple diffusion or by a carrier-mediated mechanism. Jinurea increased in a linear manner with increasing urea water levels (0-11 mmol N l-1), while Jouturea was independent of external urea concentrations. As well, Jneturea and Jout inurea were not significantly different from one another, indicating the absence of "back transport". Urea analogs and transport inhibitors added to the water did not have any consistent effect on unidirectional urea flux. These results demonstrate that ammonia and urea excretion rates and sites of excretion in tidepool sculpins are very similar to those found in other marine and freshwater teleosts. Urea and ammonia may play a role in osmoregulation as excretion rates and tissue levels were influenced by changes in water salinity. Finally, we found no evidence for a specific urea carrier; branchial urea excretion is likely dependent on simple diffusion.

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalFish Physiology and Biochemistry
Volume14
Issue number2
DOIs
StatePublished - Apr 1 1995

Fingerprint

Salinity
Ammonia
excretion
urea
Urea
ammonia
salinity
Water
Seawater
effect
Oligocottus maculosus
gills
seawater
fold
Osmoregulation
osmoregulation
Tides
tidal cycle
water salinity
Water levels

Keywords

  • gills
  • marine teleost
  • nitrogenous waste products
  • osmoregulation
  • unidirectional urea flux

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Aquatic Science

Cite this

Ammonia and urea excretion in the tidepool sculpin (Oligocottus maculosus) : sites of excretion, effects of reduced salinity and mechanisms of urea transport. / Wright, P. A.; Part, P.; Wood, C. M.

In: Fish Physiology and Biochemistry, Vol. 14, No. 2, 01.04.1995, p. 111-123.

Research output: Contribution to journalArticle

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abstract = "Tidepool sculpins live in a variable environment where water temperature, salinity, gas tensions, and pH can change considerably with the daily tide cycle. Tidepool sculpins are primarily ammoniotelic, with 8-17{\%} of nitrogen wastes excreted as urea. The majority of net ammonia (Jnetamm; 85{\%}) and urea (Jneturea; 74{\%}) excretion occurred across the gill, with the remainder excreted across the skin, the kidney, and/or gut. Acute (2h) exposure to 50{\%} seawater significantly increased Jneturea (2.8-fold), but reduced Jnetamm (3.5-fold). In fish exposed to 50{\%} seawater for 1 week, Jneturea returned to control values, but Jnetamm remained slightly depressed. Unidirectional urea influx (Jinurea) and efflux (Jouturea) were measured using14C-urea to determine if urea was excreted across the gills by simple diffusion or by a carrier-mediated mechanism. Jinurea increased in a linear manner with increasing urea water levels (0-11 mmol N l-1), while Jouturea was independent of external urea concentrations. As well, Jneturea and Jout inurea were not significantly different from one another, indicating the absence of {"}back transport{"}. Urea analogs and transport inhibitors added to the water did not have any consistent effect on unidirectional urea flux. These results demonstrate that ammonia and urea excretion rates and sites of excretion in tidepool sculpins are very similar to those found in other marine and freshwater teleosts. Urea and ammonia may play a role in osmoregulation as excretion rates and tissue levels were influenced by changes in water salinity. Finally, we found no evidence for a specific urea carrier; branchial urea excretion is likely dependent on simple diffusion.",
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