Amino acid transport in blood cells II. Patterns of transport of some amino acids in mammalian reticulocytes and mature red blood cells

Adel A Yunis, Grace K. Arimura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Amino acid transport and its cationic requirements have been studied in mammalian reticulocytes and mature red blood cells. Sodium is required for the concentrative uptake of AIB (alpha-aminoisobutyric acid-1-C14) glycine and alanine by human and rat reticulocytes, whereas the concentrative uptake of ACPC (aminocyclopentane-carboxylic acid-C14), histidine, and lysine was readily demonstrable without added sodium. Amino, acid uptake was markedly inhibited by the sulfhydryl reagents p-hydroxymercuribenzoate and N-ethyl-maleimide. Puromycin completely blocked the uptake of alanine and lysine into protein without interfering with the intracellular accumulation of these amino acids. The capacity to concentrate amino acids against a chemical gradient is markedly reduced or absent in the mature red cell. Some possible explanations for this metabolic change are discussed.

Original languageEnglish (US)
Pages (from-to)177-186
Number of pages10
JournalThe Journal of laboratory and clinical medicine
Volume66
Issue number2
StatePublished - Aug 1965

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Reticulocytes
Blood Cells
Blood
Erythrocytes
Cells
Amino Acids
Alanine
Lysine
Sodium
Puromycin
Sulfhydryl Reagents
Carboxylic Acids
Histidine
Glycine
Rats
Proteins

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)
  • Pathology and Forensic Medicine
  • Physiology (medical)

Cite this

Amino acid transport in blood cells II. Patterns of transport of some amino acids in mammalian reticulocytes and mature red blood cells. / Yunis, Adel A; Arimura, Grace K.

In: The Journal of laboratory and clinical medicine, Vol. 66, No. 2, 08.1965, p. 177-186.

Research output: Contribution to journalArticle

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