Amino acid transport and its cationic requirements have been studied in mammalian reticulocytes and mature red blood cells. Sodium is required for the concentrative uptake of AIB (alpha-aminoisobutyric acid-1-C14) glycine and alanine by human and rat reticulocytes, whereas the concentrative uptake of ACPC (aminocyclopentane-carboxylic acid-C14), histidine, and lysine was readily demonstrable without added sodium. Amino, acid uptake was markedly inhibited by the sulfhydryl reagents p-hydroxymercuribenzoate and N-ethyl-maleimide. Puromycin completely blocked the uptake of alanine and lysine into protein without interfering with the intracellular accumulation of these amino acids. The capacity to concentrate amino acids against a chemical gradient is markedly reduced or absent in the mature red cell. Some possible explanations for this metabolic change are discussed.
|Original language||English (US)|
|Number of pages||10|
|Journal||The Journal of Laboratory and Clinical Medicine|
|State||Published - Aug 1 1965|
ASJC Scopus subject areas
- Pathology and Forensic Medicine