Amino acid transport in blood cells II. Patterns of transport of some amino acids in mammalian reticulocytes and mature red blood cells

Adel A. Yunis, Grace K. Arimura

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Abstract

Amino acid transport and its cationic requirements have been studied in mammalian reticulocytes and mature red blood cells. Sodium is required for the concentrative uptake of AIB (alpha-aminoisobutyric acid-1-C14) glycine and alanine by human and rat reticulocytes, whereas the concentrative uptake of ACPC (aminocyclopentane-carboxylic acid-C14), histidine, and lysine was readily demonstrable without added sodium. Amino, acid uptake was markedly inhibited by the sulfhydryl reagents p-hydroxymercuribenzoate and N-ethyl-maleimide. Puromycin completely blocked the uptake of alanine and lysine into protein without interfering with the intracellular accumulation of these amino acids. The capacity to concentrate amino acids against a chemical gradient is markedly reduced or absent in the mature red cell. Some possible explanations for this metabolic change are discussed.

Original languageEnglish (US)
Pages (from-to)177-186
Number of pages10
JournalThe Journal of Laboratory and Clinical Medicine
Volume66
Issue number2
StatePublished - Aug 1 1965

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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