Amino acid acceptor activity of tRNA-C-C-C-A

Arthur H. Kirschenbaum; Murray P Deutscher

doi:10.1016/0006-291X(76)91136-0

Amino acid acceptor activity of tRNA-C-C-C-A

Arthur H. Kirschenbaum, Murray P Deutscher

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5 Scopus citations

Abstract

Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.

Original language	English
Pages (from-to)	258-264
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(76)91136-0
State	Published - May 3 1976
Externally published	Yes

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Kirschenbaum, A. H., & Deutscher, M. P. (1976). Amino acid acceptor activity of tRNA-C-C-C-A. Biochemical and Biophysical Research Communications, 70(1), 258-264. https://doi.org/10.1016/0006-291X(76)91136-0

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N2 - Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.

AB - Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.

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10.1016/0006-291X(76)91136-0