Amino acid acceptor activity of tRNA-C-C-C-A

Arthur H. Kirschenbaum, Murray P Deutscher

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.

Original languageEnglish
Pages (from-to)258-264
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume70
Issue number1
DOIs
StatePublished - May 3 1976
Externally publishedYes

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Transfer RNA
Amino Acids
Aminoacylation
Cytidine Monophosphate
Molecules
Adenosine Monophosphate
Liver
Rabbits
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Amino acid acceptor activity of tRNA-C-C-C-A. / Kirschenbaum, Arthur H.; Deutscher, Murray P.

In: Biochemical and Biophysical Research Communications, Vol. 70, No. 1, 03.05.1976, p. 258-264.

Research output: Contribution to journalArticle

Kirschenbaum, Arthur H. ; Deutscher, Murray P. / Amino acid acceptor activity of tRNA-C-C-C-A. In: Biochemical and Biophysical Research Communications. 1976 ; Vol. 70, No. 1. pp. 258-264.
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