Abstract
Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.
| Original language | English |
|---|---|
| Pages (from-to) | 258-264 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 70 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 3 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Amino acid acceptor activity of tRNA-C-C-C-A. / Kirschenbaum, Arthur H.; Deutscher, Murray P.
In: Biochemical and Biophysical Research Communications, Vol. 70, No. 1, 03.05.1976, p. 258-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amino acid acceptor activity of tRNA-C-C-C-A
AU - Kirschenbaum, Arthur H.
AU - Deutscher, Murray P
PY - 1976/5/3
Y1 - 1976/5/3
N2 - Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.
AB - Rabbit liver tRNA nucleotidyltransferase was used to synthesize modified tRNA molecules containing an additional CMP residue at the 3′ terminus. In the first step tRNA-C-C was converted to tRNA-C-C-C by a minor activity of the purified enzyme. In the second step the lengthened molecules were converted to tRNA-C-C-C-A. AMP addition to tRNA-C-C-C occurred at about 50% the rate with tRNA-C-C. Aminoacylation studies indicated that tRNA-C-C-C-A was active for acceptance of at least 12 amino acids.
UR - http://www.scopus.com/inward/record.url?scp=0017066529&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017066529&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(76)91136-0
DO - 10.1016/0006-291X(76)91136-0
M3 - Article
C2 - 1275940
AN - SCOPUS:0017066529
VL - 70
SP - 258
EP - 264
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -