AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice

Anna C. Pulliam, M. Joe Hobson, Samantha L. Ciccone, Yan Li, Shi Chen, Edward F. Srour, Feng-Chun Yang, Hal E. Broxmeyer, D. Wade Clapp

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Fanconi anemia (FA) is a heterogeneous inherited disorder characterized by a progressive bone marrow (BM) failure and susceptibility to myeloid leukemia. Genetic correction using gene-transfer technology is one potential therapy. A major hurdle in applying this technology in FA patients is the inability of granulocyte colony-stimulating factor (G-CSF) to mobilize sufficient numbers of hematopoietic stem (HSC)/progenitor cells (HPC) from the BM to the peripheral blood. Whether the low number of CD34+ cells is a result of BM hypoplasia or an inability of G-CSF to adequately mobilize FA HSC/HPC remains incompletely understood. Here we use competitive repopulation of lethally irradiated primary and secondary recipients to show that in two murine models of FA, AMD3100 synergizes with G-CSF resulting in a mobilization of HSC, whereas G-CSF alone fails to mobilize stem cells even in the absence of hypoplasia.

Original languageEnglish (US)
Pages (from-to)1084-1090
Number of pages7
JournalExperimental Hematology
Volume36
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

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Fanconi Anemia
Granulocyte Colony-Stimulating Factor
Knockout Mice
Stem Cells
Bone Marrow
Technology Transfer
Myeloid Leukemia
Cell Count
Technology
JM 3100
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Pulliam, A. C., Hobson, M. J., Ciccone, S. L., Li, Y., Chen, S., Srour, E. F., ... Clapp, D. W. (2008). AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice. Experimental Hematology, 36(9), 1084-1090. https://doi.org/10.1016/j.exphem.2008.03.016

AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice. / Pulliam, Anna C.; Hobson, M. Joe; Ciccone, Samantha L.; Li, Yan; Chen, Shi; Srour, Edward F.; Yang, Feng-Chun; Broxmeyer, Hal E.; Clapp, D. Wade.

In: Experimental Hematology, Vol. 36, No. 9, 09.2008, p. 1084-1090.

Research output: Contribution to journalArticle

Pulliam, AC, Hobson, MJ, Ciccone, SL, Li, Y, Chen, S, Srour, EF, Yang, F-C, Broxmeyer, HE & Clapp, DW 2008, 'AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice', Experimental Hematology, vol. 36, no. 9, pp. 1084-1090. https://doi.org/10.1016/j.exphem.2008.03.016
Pulliam, Anna C. ; Hobson, M. Joe ; Ciccone, Samantha L. ; Li, Yan ; Chen, Shi ; Srour, Edward F. ; Yang, Feng-Chun ; Broxmeyer, Hal E. ; Clapp, D. Wade. / AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice. In: Experimental Hematology. 2008 ; Vol. 36, No. 9. pp. 1084-1090.
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