AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice

Anna C. Pulliam, M. Joe Hobson, Samantha L. Ciccone, Yan Li, Shi Chen, Edward F. Srour, Feng-Chun Yang, Hal E. Broxmeyer, D. Wade Clapp

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Fanconi anemia (FA) is a heterogeneous inherited disorder characterized by a progressive bone marrow (BM) failure and susceptibility to myeloid leukemia. Genetic correction using gene-transfer technology is one potential therapy. A major hurdle in applying this technology in FA patients is the inability of granulocyte colony-stimulating factor (G-CSF) to mobilize sufficient numbers of hematopoietic stem (HSC)/progenitor cells (HPC) from the BM to the peripheral blood. Whether the low number of CD34+ cells is a result of BM hypoplasia or an inability of G-CSF to adequately mobilize FA HSC/HPC remains incompletely understood. Here we use competitive repopulation of lethally irradiated primary and secondary recipients to show that in two murine models of FA, AMD3100 synergizes with G-CSF resulting in a mobilization of HSC, whereas G-CSF alone fails to mobilize stem cells even in the absence of hypoplasia.

Original languageEnglish (US)
Pages (from-to)1084-1090
Number of pages7
JournalExperimental Hematology
Volume36
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Pulliam, A. C., Hobson, M. J., Ciccone, S. L., Li, Y., Chen, S., Srour, E. F., Yang, F-C., Broxmeyer, H. E., & Clapp, D. W. (2008). AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice. Experimental Hematology, 36(9), 1084-1090. https://doi.org/10.1016/j.exphem.2008.03.016