Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium

Toshiyuki Arai, Susan A. Kelly, Matthew L. Brengman, Manabu Takano, Elise H. Smith, Pascal Goldschmidt-Clermont, Gregory B. Bulkley

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Proinflammatory cytokines upregulate endothelial adhesion molecule expression, thereby initiating the microvascular inflammatory response. We re-evaluated the reported role of reactive oxygen metabolites (ROMs) in signalling upregulation of intercellular adhesion molecule 1 (ICAM-1) on endothelial cells by tumour necrosis factor α (TNF-α) in vitro. TNF-α upregulation of endothelial-cell ICAM-1 expression was inhibited by the cell-permeable antioxidants, or by the adenovirus-mediated intracellular overexpression of Cu,Zn-superoxide dismutase, but not by the exogenous (extracellular) administration of the cell-impermeable antioxidants, superoxide dismutase and/or catalase. This ICAM-1 upregulation was also inhibited by inhibitors of NADH dehydrogenase, cytochrome bc1 complex and NADPH oxidase. However, a measurable increase in net cellular ROM generation in response to TNF-α was not seen using four disparate sensitive ROM assays. Moreover, the stimulation of exogenous or endogenous ROM generation did not upregulate ICAM-1, nor enhance ICAM-1 upregulation by TNF-α. These findings suggest that an ambient background flux of ROMs, generated intracellularly, but not their net incremental generation, is necessary for TNF-α to induce ICAM-1 expression in endothelium in vitro.

Original languageEnglish
Pages (from-to)853-861
Number of pages9
JournalBiochemical Journal
Volume331
Issue number3
StatePublished - May 1 1998
Externally publishedYes

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Cohort Effect
Intercellular Adhesion Molecule-1
Oxidants
Endothelium
Metabolites
Up-Regulation
Tumor Necrosis Factor-alpha
Oxygen
Endothelial cells
Endothelial Cells
Antioxidants
NADH Dehydrogenase
NADPH Oxidase
Electron Transport Complex III
Cell Adhesion Molecules
Adenoviridae
Catalase
Superoxide Dismutase
Assays
Adhesion

ASJC Scopus subject areas

  • Biochemistry

Cite this

Arai, T., Kelly, S. A., Brengman, M. L., Takano, M., Smith, E. H., Goldschmidt-Clermont, P., & Bulkley, G. B. (1998). Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium. Biochemical Journal, 331(3), 853-861.

Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium. / Arai, Toshiyuki; Kelly, Susan A.; Brengman, Matthew L.; Takano, Manabu; Smith, Elise H.; Goldschmidt-Clermont, Pascal; Bulkley, Gregory B.

In: Biochemical Journal, Vol. 331, No. 3, 01.05.1998, p. 853-861.

Research output: Contribution to journalArticle

Arai, T, Kelly, SA, Brengman, ML, Takano, M, Smith, EH, Goldschmidt-Clermont, P & Bulkley, GB 1998, 'Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium', Biochemical Journal, vol. 331, no. 3, pp. 853-861.
Arai T, Kelly SA, Brengman ML, Takano M, Smith EH, Goldschmidt-Clermont P et al. Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium. Biochemical Journal. 1998 May 1;331(3):853-861.
Arai, Toshiyuki ; Kelly, Susan A. ; Brengman, Matthew L. ; Takano, Manabu ; Smith, Elise H. ; Goldschmidt-Clermont, Pascal ; Bulkley, Gregory B. / Ambient but not incremental oxidant generation effects intercellular adhesion molecule 1 induction by tumour necrosis factor α in endothelium. In: Biochemical Journal. 1998 ; Vol. 331, No. 3. pp. 853-861.
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