Alzheimer's disease cells exhibit defective repair of alkylating agent-induced DNA damage

S. H. Robison, J. S. Munzer, R. Tandan, Walter G Bradley

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The most common cause of senile and presenile dementia is Alzheimer's disease, a disorder with an undetermined cause. A number of studies have indicated that neurons from patients with Alzheimer's disease have decreased ribonucleic acid levels and reduced protein synthesis. Recent studies using lymphoblasts from patients with Alzheimer's disease have indicated that these cells are more sensitive to deoxyribonucleic acid (DNA)-alkylating agents. We have used cell survival, unscheduled DNA synthesis, and alkaline elution to assess the capacity for DNA repair in skin fibroblasts from normal control subjects, control subjects with central nervous system disease, and patients with Alzheimer's disease. Our results indicate that the Alzheimer's disease cells, unlike normal cells, fail to repair methyl-methane sulfonate-induced DNA damage. Both normal and Alzheimer's disease cells are able to ameliorate the effects of ultraviolet light. These results indicate that a specific pathway for DNA repair is affected in Alzheimer's disease. The repair defect may be related to the cause of the disease or may be the cause of the disease.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalAnnals of Neurology
Volume21
Issue number3
StatePublished - Jan 1 1987
Externally publishedYes

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Alkylating Agents
Alzheimer Disease
DNA
Central Nervous System Diseases
Methane
Ultraviolet Rays
Cell Survival
Fibroblasts
RNA
Neurons
Skin
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alzheimer's disease cells exhibit defective repair of alkylating agent-induced DNA damage. / Robison, S. H.; Munzer, J. S.; Tandan, R.; Bradley, Walter G.

In: Annals of Neurology, Vol. 21, No. 3, 01.01.1987, p. 250-258.

Research output: Contribution to journalArticle

Robison, S. H. ; Munzer, J. S. ; Tandan, R. ; Bradley, Walter G. / Alzheimer's disease cells exhibit defective repair of alkylating agent-induced DNA damage. In: Annals of Neurology. 1987 ; Vol. 21, No. 3. pp. 250-258.
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