Aluminum Increases Agonist‐Stimulated Cyclic AMP Production in Rat Cerebral Cortical Slices

Gail V.W. Johnson, Xiaohua Li, Richard S. Jope

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The effects of AlCl3 on basal and stimulated cyclic AMP production in rat cerebral cortical slices were studied. AlCl3 (10-250 μM) had no effect on the cyclic AMP concentration in the absence of drugs that stimulate the synthesis of cyclic AMP. 2-Chloroadenosine (25-200 μM) significantly stimulated the synthesis of cyclic AMP in a concentration-dependent manner, and AlCl3 significantly potentiated this response at 50 and 100 μM 2-chloroadenosine. This effect of AlCl3 was dependent on preexposure of the slices to AlCl3 before addition of the agonist. The potentiation by AlCl3 of the 2-chloroadenosine-induced increase in cyclic AMP level was concentration dependent, with significant enhancement by 100 (142% of the control) and 250 (150% of the control) μM AlCl3. Lower concentrations of AlCl3 had no significant effect on the production of cyclic AMP stimulated by 2-chloroadenosine. AlCl3 also potentiated the isoproterenol-induced increase in cyclic AMP production. Forskolin-induced production of cyclic AMP was unaltered by the presence of AlCl3. These results demonstrate that AlCl3 can potentiate agonist-stimulated cyclic AMP production in a whole-cell brain preparation without the addition of fluoride. This may account for the previously reported aluminum-induced increase in cyclic AMP concentrations in rat brain in vivo.

Original languageEnglish (US)
Pages (from-to)258-263
Number of pages6
JournalJournal of neurochemistry
Volume53
Issue number1
DOIs
StatePublished - Jul 1989
Externally publishedYes

Keywords

  • 2‐Chloroadenosine
  • Aluminum
  • Cyclic AMP

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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