TY - JOUR
T1 - Aluminum chloride stimulates the release of endogenous glutamate, taurine and adenosine from cultured rat cortical astrocytes
AU - Albrecht, Jan
AU - Simmons, Martha
AU - Dutton, Gary R.
AU - Norenberg, Michael D.
N1 - Funding Information:
The authors would like to thank Marilynn Kirkpa-trick and Kathryn Andrews for manuscriptp reparation. This work was supportedb y the VeteranA dministration and an NIH Grant DK 38153,a grant from the University of Miami (M.D.N.), by NIH Grant NS 20632 (G.R.D.), and the Life and Health InsuranceM edical ResearchF und (M.L.S.).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1991/6/10
Y1 - 1991/6/10
N2 - Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.
AB - Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.
KW - Aluminum toxicity
KW - Cerebral cortex
KW - Cultured astrocyte
KW - Taurine
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U2 - 10.1016/0304-3940(91)90905-9
DO - 10.1016/0304-3940(91)90905-9
M3 - Article
C2 - 1679221
AN - SCOPUS:0025922307
VL - 127
SP - 105
EP - 107
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -