Aluminum chloride stimulates the release of endogenous glutamate, taurine and adenosine from cultured rat cortical astrocytes

Jan Albrecht; Martha Simmons; Gary R. Dutton; Michael D. Norenberg

doi:10.1016/0304-3940(91)90905-9

Aluminum chloride stimulates the release of endogenous glutamate, taurine and adenosine from cultured rat cortical astrocytes

Jan Albrecht, Martha Simmons, Gary R. Dutton, Michael D. Norenberg

Pathology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl₃, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl₃ stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl₃ enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.

Original language	English (US)
Pages (from-to)	105-107
Number of pages	3
Journal	Neuroscience Letters
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90905-9
State	Published - Jun 10 1991

Keywords

Aluminum toxicity
Cerebral cortex
Cultured astrocyte
Taurine

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(91)90905-9

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@article{384f35763cee45f3926cfbc9317c85bd,

title = "Aluminum chloride stimulates the release of endogenous glutamate, taurine and adenosine from cultured rat cortical astrocytes",

abstract = "Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.",

keywords = "Aluminum toxicity, Cerebral cortex, Cultured astrocyte, Taurine",

author = "Jan Albrecht and Martha Simmons and Dutton, {Gary R.} and Norenberg, {Michael D.}",

note = "Funding Information: The authors would like to thank Marilynn Kirkpa-trick and Kathryn Andrews for manuscriptp reparation. This work was supportedb y the VeteranA dministration and an NIH Grant DK 38153,a grant from the University of Miami (M.D.N.), by NIH Grant NS 20632 (G.R.D.), and the Life and Health InsuranceM edical ResearchF und (M.L.S.). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1991",

month = jun,

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doi = "10.1016/0304-3940(91)90905-9",

language = "English (US)",

volume = "127",

pages = "105--107",

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AU - Albrecht, Jan

AU - Simmons, Martha

AU - Dutton, Gary R.

AU - Norenberg, Michael D.

N1 - Funding Information: The authors would like to thank Marilynn Kirkpa-trick and Kathryn Andrews for manuscriptp reparation. This work was supportedb y the VeteranA dministration and an NIH Grant DK 38153,a grant from the University of Miami (M.D.N.), by NIH Grant NS 20632 (G.R.D.), and the Life and Health InsuranceM edical ResearchF und (M.L.S.). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/6/10

Y1 - 1991/6/10

N2 - Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.

AB - Primary astrocyte cultures derived from neonatal rat cerebral cortex were treated for 5 min with 0.5 mM or 5.0 mM AlCl3, and the incubation medium was analyzed by HPLC for the content of released glutamate (Glu), taurine (Tau), serine (Ser) and the nucleoside adenosine (Ade). At 0.5 mM, AlCl3 stimulated Tau release to about 170% of basal levels, but did not affect the release of the other compounds. Treatment with 5.0 mM AlCl3 enhanced the release of Tau, Glu and Ade, to 800%, 1000% and 250%, respectively, but decreased the release of Ser to 70% compared to basal levels. The enhanced release of these neuroactive compounds from astrocytes may contribute to changes in neural transmission known to accompany exposure to aluminum.

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